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Point-of-care detection of Monkeypox virus clades using high-performance upconversion nanoparticle-based lateral flow assay

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Abstract

There is an urgent need for a point-of-care testing (POCT) method in developing and underserved regions to distinguish between two Monkeypox virus (MPXV) clades, given their varying transmissibility and clinical manifestations. In this paper, we target the specific complement protein gene fragment of two MPXV clades and construct a high-performance upconversion nanoparticles–based lateral flow assay (UCNPs-based LFA) with double T-lines and a shared C-line. This enables qualitative and quantitative dual-mode detection when combined with a smartphone and a benchtop fluorescence analyzer. The developed LFA exhibits stable performance, convenient operation, rapid readout (within 8 min), and a much lower limit of detection (LOD) (~ pM level) compared to existing POCT methods. The proposed detection platform demonstrates significant potential for pathogen diagnosis using a POCT approach.

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Funding

This work was supported by the Opening Research Fund from Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research, College of Stomatology, Xi’an Jiaotong University (2019LHM-KFKT005); the General Financial Grant from the China Postdoctoral Science Foundation (2020M673418); the Natural Science Foundation of the Anhui Higher Education Institutions of China (KJ2021ZD0150); and the National Natural Science Foundation of China (61904143).

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Authors and Affiliations

  1. School of Materials and Chemical Engineering, Xi’an Technological University, Xi’an, 710021, People’s Republic of China

    Birui Jin, Chuan Ma & Chuyao Zhang

  2. Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research, College of Stomatology, Xi’an Jiaotong University, Xi’an, People’s Republic of China

    Zedong Li

  3. Bioinspired Engineering and Biomechanics Center (BEBC), Xi’an Jiaotong University, Xi’an, 710049, People’s Republic of China

    Birui Jin, Huiling Yin, Guoxu Zhao & Zedong Li

  4. State Key Laboratory of Digital Medical Engineering, School of Biomedical Engineering, Hainan University, Haikou, 570228, People’s Republic of China

    Guoxu Zhao

  5. Suzhou DiYinAn Biotech Co., Ltd., Suzhou Innovation Center for Life Science and Technology, Suzhou, 215129, People’s Republic of China

    Jie Hu

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  1. Birui Jin
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Correspondence to Jie Hu or Zedong Li.

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Jin, B., Ma, C., Zhang, C. et al. Point-of-care detection of Monkeypox virus clades using high-performance upconversion nanoparticle-based lateral flow assay. Microchim Acta 191, 177 (2024). https://doi.org/10.1007/s00604-024-06241-3

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