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Novel RNA genosensor based on highly stable gold nanoparticles decorated phosphorene nanohybrid with graphene for highly sensitive and low-cost electrochemical detection of coconut cadang-cadang viroid

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Abstract

Coconut cadang-cadang viroid (CCCVd) is an infectious single-stranded RNA (ssRNA) pathogen, which leads directly to the death of a large number of coconut palm trees and heavy economic loss to coconut farmers. Herein, a novel electrochemical impedance RNA genosensor is presented based on highly stable gold nanoparticles (AuNPs) decorated phosphorene (BP) nanohybrid with graphene (Gr) for highly sensitive, low-cost, and label-free detection of CCCVd. BP-AuNPs are environmentally friendly prepared by ultrasonic-assisted liquid-phase exfoliation of black phosphorus, accompanying direct reduction of chloroauric acid. Gr/BP-AuNPs are facilely prepared by the in situ growth of AuNPs onto the BP surface and its nanohybrid with Gr to improve environmental stability of BP. Gr/BP-AuNP-based RNA genosensor is fabricated by immobilizing the thiol-functionalized single-stranded DNA (ssDNA) oligonucleotide probe onto the surface of Gr/BP-AuNP-modified glassy carbon electrode via gold-thiol interactions, which served as an electrochemical genosensing platform for the label-free impedance detection of CCCVd by hybridization between the functionalized ssDNA probe and the complementary CCCVd ssRNA sequence in a wide linear range from 1.0 × 10−11 to 1.0 × 10−7 M with a low limit of detection of 2.8 × 10−12 M. This work supplies an experimental support and theoretical direction for the fabrication of RNA biosensors based on graphene-like materials and potential application for a specific diagnosis of plant RNA viral disease in Arecaceae planting industry.

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Funding

The work is supported by the National Natural Science Foundation of China (51962007, 31960449), Training Project of High-Level and High-Skilled Leading Talents of Jiangxi Province; the Natural Science Foundation of Jiangxi Province (20192ACBL21015); and the Jiangxi Provincial Department of Education (GJJ200461).

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Authors and Affiliations

  1. Department of Biochemistry and Molecular Biology, College of Bioscience and Bioengineering, Jiangxi Agricultural University, Nanchang, 330045, People’s Republic of China

    Yutang Wang, Yihua Wang & Jianbo Song

  2. Institute of Functional Materials and Agricultural Applied Chemistry, Jiangxi Agricultural University, Nanchang, 330045, People’s Republic of China

    Yutang Wang, Wenqi Wang, Xinyu Lu, Tao Chen, Yangping Wen, Jiaqi Hu & Xiaoqiang Wang

  3. Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education, Jiangxi Agricultural University, Nanchang, 330045, People’s Republic of China

    Yangping Wen

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  1. Yutang Wang
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  4. Tao Chen
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Contributions

YuWa: Investigation, data processing, formal analysis, writing (original draft), writing—review and editing. WW: Investigation, data processing, formal analysis. XL: Conceptualization, investigation, methodology. TC: Methodology, investigation. YiWa: Project administration, Methodology, Supervision. YaWe: Writing (review and editing), project administration, funding acquisition. JH: Project administration, funding acquisition. JS: Project administration, methodology, supervision. XW: Project administration, Funding acquisition.

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Correspondence to Yihua Wang or Yangping Wen.

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Wang, Y., Wang, W., Lu, X. et al. Novel RNA genosensor based on highly stable gold nanoparticles decorated phosphorene nanohybrid with graphene for highly sensitive and low-cost electrochemical detection of coconut cadang-cadang viroid. Microchim Acta 191, 52 (2024). https://doi.org/10.1007/s00604-023-06130-1

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