Abstract
A poly(n-butyl acrylate)-gold-MXene quantum dots (PBA-Au-MXene QD) nanocomposite-based biosensor is presented that is modified by unique antisense single-stranded DNA (ssDNA) and uses the electrochemical detection methods of DPV, CV, and EIS to early detect miR-122 as a breast cancer biomarker in real clinical samples. This fabrication method is based on advanced nanotechnology, at which a poly(n-butyl acrylate) (PBA) as a non-conductive polymer transforms into a conductive composite by incorporating Au-MXene QD. This biosensor had a limit of detection (LOD) of 0.8 zM and a linear range from 0.001 aM to 1000 nM, making it capable of detecting the low concentrations of miR-122 in patient samples. Moreover, it allows approximately 100% sensitivity and 100% specificity for miR-122 without extraction. The synthesis and detection characteristics were evaluated by different complementary tests such as AFM, FTIR, TEM, and FESEM. This new biosensor can have a high potential in clinical applications to detect breast cancer early and hence improve patient outcomes.
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Mohammadi M, Goodarzi M, Jaafari M, Mirzaei H, Mirzaei H (2016) Circulating microRNA: a new candidate for diagnostic biomarker in neuroblastoma. Cancer Gene Ther 23(11):371–372
Wozniak MB, Scelo G, Muller DC, Mukeria A, Zaridze D, Brennan P (2015) Circulating microRNAs as non-invasive biomarkers for early detection of non-small-cell lung cancer. PLoS ONE 10(5):e0125026
Saleh AA, Soliman SE, Habib MSE-d, Gohar SF, Abo-Zeid GS (2019) Potential value of circulatory microRNA122 gene expression as a prognostic and metastatic prediction marker for breast cancer. Mol Biol Rep 46:2809–2818
Fong MY, Zhou W, Liu L, Alontaga AY, Chandra M, Ashby J et al (2015) Breast-cancer-secreted miR-122 reprograms glucose metabolism in premetastatic niche to promote metastasis. Nat Cell Biol 17(2):183–194
El-Khoury V, Pierson S, Kaoma T, Bernardin F, Berchem G (2016) Assessing cellular and circulating miRNA recovery: the impact of the RNA isolation method and the quantity of input material. Sci Rep 6(1):19529
El Aamri M, Yammouri G, Mohammadi H, Amine A, Korri-Youssoufi H (2020) Electrochemical biosensors for detection of microRNA as a cancer biomarker: Pros and cons. Biosensors 10(11):186
Hatamluyi B, Lorestani F, Es’haghi Z (2018) Au/Pd@ rGO nanocomposite decorated with poly (L-cysteine) as a probe for simultaneous sensitive electrochemical determination of anticancer drugs, Ifosfamide and Etoposide. Biosens Bioelectron 120:22–29
Mittal S, Kaur H, Gautam N, Mantha AK (2017) Biosensors for breast cancer diagnosis: a review of bioreceptors, biotransducers and signal amplification strategies. Biosens Bioelectron 88:217–231
Ranjbari S, Darroudi M, Hatamluyi B, Arefinia R, Aghaee-Bakhtiari SH, Rezayi M et al (2022) Application of MXene in the diagnosis and treatment of breast cancer: a critical overview. Front Bioeng Biotechnol 10:984336
Ebrahimi A, Nikokar I, Zokaei M, Bozorgzadeh E (2018) Design, development and evaluation of microRNA-199a-5p detecting electrochemical nanobiosensor with diagnostic application in Triple Negative Breast Cancer. Talanta 189:592–598
Liu L, Zhu S, Wei Y, Liu X, Jiao S, Yang J (2019) Ultrasensitive detection of miRNA-155 based on controlled fabrication of AuNPs@ MoS2 nanostructures by atomic layer deposition. Biosens Bioelectron 144:111660
Ranjbari S, Rezayi M, Arefinia R, Aghaee-Bakhtiari SH, Hatamluyi B, Pasdar A (2023) A novel electrochemical biosensor based on signal amplification of Au HFGNs/PnBA-MXene nanocomposite for the detection of miRNA-122 as a biomarker of breast cancer. Talanta 255:124247
Miao P, Wang B, Yu Z, Zhao J, Tang Y (2015) Ultrasensitive electrochemical detection of microRNA with star trigon structure and endonuclease mediated signal amplification. Biosens Bioelectron 63:365–370
Meinderink D, Orive A, Grundmeier G (2018) Electrodeposition of poly (acrylic acid) on stainless steel with enhanced adhesion properties. Surf Interface Anal 50(11):1224–1229
Smith DA, Newbury LJ, Drago G, Bowen T, Redman JE (2017) Electrochemical detection of urinary microRNAs via sulfonamide-bound antisense hybridisation. Sens Actuators, B Chem 253:335–341
Khodadoust A, Nasirizadeh N, Seyfati SM, Taheri RA, Ghanei M, Bagheri H (2023) High-performance strategy for the construction of electrochemical biosensor for simultaneous detection of miRNA-141 and miRNA-21 as lung cancer biomarkers. Talanta 252:123863
Mahmood M, Rasheed A, Ayman I, Rasheed T, Munir S, Ajmal S et al (2021) Synthesis of ultrathin MnO2 nanowire-intercalated 2D-MXenes for high-performance hybrid supercapacitors. Energy Fuels 35(4):3469–3478
Lee E, VahidMohammadi A, Prorok BC, Yoon YS, Beidaghi M, Kim D-J (2017) Room temperature gas sensing of two-dimensional titanium carbide (MXene). ACS Appl Mater Interfaces 9(42):37184–37190
Enríquez JMH, Lajas LAC, Alamilla RG, Alamilla PG, Handy EB, Galindo GC, Serrano LAG (2013) Synthesis of solid acid catalysts based on TiO2-SO42-and Pt/TiO2-SO42-applied in n-hexane isomerization. Open J Metal 2013(3):34–44
Khan AR, Husnain SM, Shahzad F, Mujtaba-ul-Hassan S, Mehmood M, Ahmad J et al (2019) Two-dimensional transition metal carbide (Ti 3 C 2 T x) as an efficient adsorbent to remove cesium (Cs+). Dalton Trans 48(31):11803–11812
Li Y, Sun XS (2015) Synthesis and characterization of acrylic polyols and polymers from soybean oils for pressure-sensitive adhesives. RSC Adv 5(55):44009–44017
Yılmaz O, Özkan ÇK, Yılmaz CN, Yorgancıoğlu A, Özgünay H, Karavana HA (2017) Synthesis and characterization of functional acrylic copolymers via RAFT mini-emulsion polymerization. AIP Confer Proc 1918(1):020006
Wang G (2011) Synthesis of poly (n-butyl acrylate) homopolymers by activators generated by electron transfer (AGET) ATRP using FeCl3· 6H2O/succinic acid catalyst. Iran Polym J 20(11):931–938
Oh SJ, Lee SC, Park SY (2006) Photopolymerization and photobleaching of n-butyl acrylate/fumed silica composites monitored by real time FTIR-ATR spectroscopy. Vib Spectrosc 42(2):273–277
Tsekenis G, Chatzipetrou M, Tanner J, Chatzandroulis S, Thanos D, Tsoukalas D et al (2012) Surface functionalization studies and direct laser printing of oligonucleotides toward the fabrication of a micromembrane DNA capacitive biosensor. Sens Actuators, B Chem 175:123–131
Manzano M, Viezzi S, Mazerat S, Marks RS, Vidic J (2018) Rapid and label-free electrochemical DNA biosensor for detecting hepatitis A virus. Biosens Bioelectron 100:89–95
Chen Y, Wang A-J, Yuan P-X, Luo X, Xue Y, Feng J-J (2019) Three dimensional sea-urchin-like PdAuCu nanocrystals/ferrocene-grafted-polylysine as an efficient probe to amplify the electrochemical signals for ultrasensitive immunoassay of carcinoembryonic antigen. Biosens Bioelectron 132:294–301
Zare H, Meshkat Z, Hatamluyi B, Rezayi M, Ghazvini K, Derakhshan M et al (2022) The first diagnostic test for specific detection of Mycobacterium simiae using an electrochemical label-free DNA nanobiosensor. Talanta 238:123049
Hatamluyi B, Sadeghian R, Sany SBT, Alipourfard I, Rezayi M (2021) Dual-signaling electrochemical ratiometric strategy for simultaneous quantification of anticancer drugs. Talanta 234:122662
Hryniewicz BM, Volpe J, Bach-Toledo L, Kurpel KC, Deller AE, Soares AL et al (2022) Development of polypyrrole (nano) structures decorated with gold nanoparticles toward immunosensing for COVID-19 serological diagnosis. Mater Today Chem 24:100817
Nguyen TQ, Breitkopf C (2018) Determination of diffusion coefficients using impedance spectroscopy data. J Electrochem Soc 165(14):E826–E831
Eskandari M, Faridbod F (2018) A printable voltammetric genosensor for tumour suppressor gene screening based on a nanocomposite of Ceria NPs–GO/nano-PANI. New J Chem 42(19):15655–15662
Lu L, Liu C, Miao W, Wang X, Guo G (2020) Ultrasensitive detection of miRNA based on efficient immobilization of probe and electrochemiluminescent quenching of Ru (bpy) 32+ by methylene blue. Anal Chim Acta 1093:52–60
Kapoor A, Ritter J, Yang RT (1990) An extended Langmuir model for adsorption of gas mixtures on heterogeneous surfaces. Langmuir 6(3):660–664
Hatamluyi B, Rezayi M, Jamehdar SA, Rizi KS, Mojarrad M, Meshkat Z et al (2022) Sensitive and specific clinically diagnosis of SARS-CoV-2 employing a novel biosensor based on boron nitride quantum dots/flower-like gold nanostructures signal amplification. Biosens Bioelectron 207:114209
Acknowledgements
We want to thank Khorasan Razavi Gas Co. for providing us with the laboratory facilities required for conducting the experiments for the present study.
Funding
This study was financially supported by (1) Ferdowsi University of Mashhad (Grant No.55634), Mashhad, Iran, and (2) Cancer Research Center, Shahid Beheshti University of Medical Sciences (Grant No. 29339), Tehran, Iran.
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Majid Rezayi is the corresponding author who died while conducting the research.
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Ranjbari, S., Hatamluyi, B., Aghaee-Bakhtiari, S.H. et al. A label-free electrochemical biosensor based on PBA-Au-MXene QD for miR-122 detection in serum samples. Microchim Acta 190, 482 (2023). https://doi.org/10.1007/s00604-023-06062-w
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DOI: https://doi.org/10.1007/s00604-023-06062-w