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Synthesis of a core–shell magnetic covalent organic framework for the enrichment and detection of aflatoxin in food using HPLC-MS/MS

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Abstract

A porous magnetic covalent organic framework, Fe3O4@TPBD-TPA (terephthalaldehyde (TPA) , N, N, N′, N′-tetrakis(p-aminophenyl)-p-phenylenediamine (TPBD)), was synthesized using the Schiff base reaction under mild reaction conditions. This adsorbent exhibited excellent adsorption performance for aflatoxins. The adsorption capacity of Fe3O4@TPBD-TPA for aflatoxins ranged from 64.4 to 84.4 mg/g. A magnetic solid-phase extraction combined with high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method based on Fe3O4@TPBD-TPA was developed for the efficient determination of four types of aflatoxins in food samples (maize, maize oil, peanut, and peanut oil). The determination coefficients (R2) were ≥0.9972. The method exhibited detection limits ranging from 0.01 to 0.06 μg/kg and spiked recoveries of 80.0 to 113.1%. The intra-day and inter-day precision were less than 6.77%, indicating good repeatability. The adsorbent showed promising prospects for the efficient enrichment of trace amounts of aflatoxins in complex food matrices.

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Funding

This study was supported by the National Natural Science Foundation of China (32372430).

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Author notes

  1. Dongyue Zhao and Xiuli Xu contributed equally to the work.

Authors and Affiliations

  1. Institute of Food Safety, Chinese Academy of Inspection & Quarantine, Beijing, 100176, China

    Dongyue Zhao, Xiuli Xu, Xiujuan Wang, Bozhou Xu & Feng Zhang

  2. School of Food Science and Engineering, Qingdao Agricultural University, Shandong, Qingdao, 266109, China

    Dongyue Zhao & Wei Wu

  3. Key Laboratory of Food Quality and Safety for State Market Regulation, Beijing, 100176, China

    Dongyue Zhao, Xiuli Xu, Xiujuan Wang, Bozhou Xu & Feng Zhang

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  1. Dongyue Zhao
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Zhao, D., Xu, X., Wang, X. et al. Synthesis of a core–shell magnetic covalent organic framework for the enrichment and detection of aflatoxin in food using HPLC-MS/MS. Microchim Acta 190, 488 (2023). https://doi.org/10.1007/s00604-023-06051-z

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