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Fluorescence detection of dopamine based on the peroxidase-like activity of Fe3O4-MWCNTs@Hemin

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Abstract

A novel Fe3O4-MWCNTs@Hemin nanocomposite was synthesized using hemin and Fe3O4 with multi-walled carbon nanotubes (MWCNTs) by one-step hydrothermal methods. The as-prepared Fe3O4-MWCNTs@Hemin nanocomposites exhibited excellent peroxidase-like activities in the activation of H2O2. The mechanisms, kinetics, and catalytic performances of Fe3O4-MWCNTs@Hemin were systematically studied. Fe3O4-MWCNTs@Hemin can oxidize dopamine (DA) to dopaquinone in the presence of H2O2, and the intermediate products dopaquinone can further react with β-naphthol to generate a highly fluorescent derivative at 415 nm excitation wavelength. Therefore, an innovative fluorescence platform for the detection of DA was developed. The fluorescence intensity increased linearly with DA concentration in the range 0.33 to 107 μM, with a low detection limit of 0.14 μM. Due to the excellent activity, substrate universality, fast response, high selectivity, and sensitivity of Fe3O4-MWCNTs@Hemin, the proposed fluorescence method was used to analyze complex biological blood samples with a satisfactory result. It demonstrated the significant potential for developing effective and dependable fluorescent analytical platforms for preserving human health.

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Funding

This work was supported by the Analysis and Testing Foundation of Kunming University of Science and Technology (grant numbers 2021T20200097 and 2021M20202118090).

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Authors and Affiliations

  1. Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, 650500, Yunnan, China

    Feijian Xiao, Yijie Wang, Qiulan Li, Dezhi Yang & Yaling Yang

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  1. Feijian Xiao
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Correspondence to Yaling Yang.

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This research was approved by the Kunming University of Science and Technology Ethic Committee (KMUST-MEC-087), and all experiments were performed in accordance with the Guideline for Experimentation of Kunming University of Science and Technology.

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The blood samples were collected from volunteers who gave informed written consent for inclusion.

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Xiao, F., Wang, Y., Li, Q. et al. Fluorescence detection of dopamine based on the peroxidase-like activity of Fe3O4-MWCNTs@Hemin. Microchim Acta 190, 259 (2023). https://doi.org/10.1007/s00604-023-05796-x

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