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Glucometer-based biosensor for the determination of ractopamine in animal-derived foods using rolling circle amplification

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Abstract

Screening for persistent organic pollutants (POPs) in food is a complex and challenging process, as POPs can be present in very low levels and can be difficult to detect. Herein, we developed an ultrasensitive biosensor based on a rolling circle amplification (RCA) platform using a glucometer to determine POP. The biosensor was constructed using gold nanoparticle probes modified with antibodies and dozens of primers, magnetic microparticle probes conjugated with haptens, and targets. After competition, RCA reactions are triggered, numerous RCA products hybridize with the ssDNA-invertase, and the target is successfully transformed into glucose. Using ractopamine as a model analyte, this strategy obtained a linear detection range of 0.038–5.00 ng mL−1 and a detection limit of 0.0158 ng mL−1, which was preliminarily verified by screening in real samples. Compared with conventional immunoassays, this biosensor utilizes the high efficiency of RCA and the portable properties of a glucometer, which effectively improves the sensitivity and simplifies the procedures using magnetic separation technology. Moreover, it has been successfully applied to ractopamine determination in animal-derived foods, revealing its potential as a promising tool for POP screening.

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Funding

This work was supported by the Natural Science Foundation of Shandong Province (ZR2020QC250), China Agriculture Research System (CARS-38), Modern Agricultural Technology Industry System of Shandong Province (SDAIT-10–10), and Key Technology Research and Development Program of Shandong (2021CXGC010809 and 2021TZXD012).

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Authors and Affiliations

  1. Institute of Food & Nutrition Science and Technology, Shandong Provincial Key Laboratory of Agro-Products Processing Technology, Key Laboratory of Novel Food Resources Processing, Ministry of Agriculture, Shandong Academy of Agricultural Sciences, Jinan, 250100, China

    Feng He, Haijie Wang, Pengfei Du, Weiting Wang, Yaobo Liu, Shoujing Wang, Yanli Ma, Yuanshang Wang & Peng Hu

  2. School of Life Sciences and Food Engineering, Hebei University of Engineering, Handan, 056038, China

    Feng He, Tengfei Li & Tianyu Tan

  3. College of Life Sciences, Yantai University, Yantai, 264005, China

    Haijie Wang

  4. College of Food Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China

    Yuanshang Wang

  5. State Key Laboratory of Biobased Material and Green Papermaking, Qilu University of Technology, Shandong Academy of Sciences, Jinan, 250353, China

    A. M. Abd El-Aty

  6. Department of Pharmacology, Faculty of Veterinary Medicine, Cairo University, Giza, 12211, Egypt

    A. M. Abd El-Aty

  7. Department of Medical Pharmacology, Medical Faculty, Ataturk University, 25240, Erzurum, Turkey

    A. M. Abd El-Aty

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  1. Feng He
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  2. Tengfei Li
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  12. A. M. Abd El-Aty
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He, F., Li, T., Wang, H. et al. Glucometer-based biosensor for the determination of ractopamine in animal-derived foods using rolling circle amplification. Microchim Acta 190, 121 (2023). https://doi.org/10.1007/s00604-023-05715-0

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