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AgPdNFs and AuNOs@GO nanocomposites for T-2 toxin detection by catalytic hairpin assembly

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Abstract

T-2 toxin is the most potent and toxic mycotoxin, produced by various Fusarium species that can potentially affect human health, and widely exists in field crops and stored grain. In this work, an electrochemical aptasensor with nonenzymatic signal amplification strategy for the detection of T-2 toxin is presented, using noble metal nanocomposites and catalytic hairpin assembly as signal amplification strategy. Silver palladium nanoflowers and gold octahedron nanoparticles@graphene oxide nanocomposites are used for synergistic amplification of electrical signals. Simultaneously, the catalytic hairpin assembly strategy based on artificial molecular technology was introduced to further amplify the signal. Under optimal conditions, T-2 toxin was measured within a linear concentration range 1 × 10−2 ~ 1 × 104 pg·mL−1 with an extremely low detection limit of 6.71 fg·mL−1. The aptasensor exhibited high sensitivity, good selectivity, satisfactory stability, and excellent reproducibility. Moreover, this method had high accuracy in detecting T-2 toxin in beer sample. The encouraging results show the potential application in foodstuff analysis.

Graphical Abstract

A dual signal amplification electrochemical biosensor for the detection of T-2 toxins was constructed, through the signal amplification of noble metal nanomaterials and CHA strategy.

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Data Availability

The raw data required to reproduce the above findings cannot be shared at thistime as the data also forms part of an ongoing study.

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Funding

This research was supported by the National Natural Science Foundation of China (Grant No. 61301037), the Program for Science and Technology Innovation Talents in Universities of Henan Province (No. 20HASTIT002), the Natural Science Foundation of Henan Province of China (No. 212300410338), and the Master’s Degree Thesis Cultivation Project of Henan University of Technology.

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Author notes

  1. Xia Lu and Long Wang contributed equally to this work.

Authors and Affiliations

  1. School of Food Science and Technology, Henan University of Technology, Zhengzhou, Henan, 450001, People’s Republic of China

    Xia Lu, Long Wang, Baoshan He, Renyong Zhao, Chunqi Bai, Yurong Zhang, Wenjie Ren, Zhiguang Suo & Yiwei Xu

  2. Coll Food Sci & Engn, Northwest A&F Univ, Yangling, 712100, Shaanxi, People’s Republic of China

    Xia Lu & Long Wang

  3. College of Electrical and Information Engineering, Zhengzhou University of Light Industry, Zhengzhou, Henan, 450002, People’s Republic of China

    Liying Jiang

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  1. Xia Lu
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Lu, X., Wang, L., He, B. et al. AgPdNFs and AuNOs@GO nanocomposites for T-2 toxin detection by catalytic hairpin assembly. Microchim Acta 190, 120 (2023). https://doi.org/10.1007/s00604-023-05700-7

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