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A ratiometric fluorescence sensor for tetracycline detection based on two fluorophores derived from Partridge tea

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Abstract

Blue fluorescent carbon dots (PCDs) were prepared by hydrothermal method with Partridge tea. The ethanol extract of Partridge tea (PEE) was found to emit red fluorescence. Thus, a novel ratiometric sensor was constructed by simply mixing the two fluorophores derived from Partridge tea. The presence of tetracycline (TET) at lower concentrations enhanced the emission peak at 508 nm of PCDs and had a negligible effect on the emission peak at 680 nm of PEE. TET at higher concentrations led to  quenching  both the fluorescence of PCDs and PEE via inner filter effect and fluorescence resonance energy transfer, separately. Good linearities for the detection of TET were obtained in the ranges 0.67 to 15.00 μM and 33.33 to 266.67 μM, with limit of detection of 0.095 μM. The sensor was successfully applied to detect TET in lake water and milk samples with good recoveries ranging from 93.27 ± 4.04% to 107.30 ± 6.16%. This study provided a simple, selective, sensitive, rapid, and environmentally friendly method of monitoring TET residues in the environment and food.

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Data Availability

The data that support the findings of this study are available from the authors upon reasonable request.

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Funding

This research received financial support from the Natural Science Foundation of Hainan Province (No. 222RC547) and Hubei Province (No. 2022CFB964) and the Starting Research Fund from the Hainan University (No. KYQD(ZR)19106) and Xiangyang Central Hospital (No. RC202002).

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Author notes

  1. Lu-Shuang Li and He-Wang Liang contributed to this work equally.

Authors and Affiliations

  1. Key Laboratory of Tropical Biological Resources of Ministry of Education, School of Pharmaceutical Sciences, Hainan University, Haikou, 570228, China

    Lu-Shuang Li, Su Li, Ying-Xia Zhang & Yan-Ting Song

  2. Xiangyang Central Hospital, affiliated hospital of Hubei University of Arts & Science, Xiangyang, 441021, China

    He-Wang Liang, Cong-Ting Wu, Wei Gong & Jing Li

Authors
  1. Lu-Shuang Li
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  2. He-Wang Liang
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  3. Cong-Ting Wu
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  4. Su Li
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  5. Ying-Xia Zhang
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  6. Yan-Ting Song
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  7. Wei Gong
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  8. Jing Li
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Contributions

Lu-Shuang Li: conceptualization, methodology, writing—original draft, funding acquisition. He-Wang Liang: conceptualization, methodology. Cong-Ting Wu: methodology, investigation. Su Li: methodology. Ying-Xia Zhang: data curation, software. Yan-Ting Song: writing—review and editing. Wei Gong: conceptualization, writing—review and revision. Jing Li: conceptualization, supervision, data curation, funding acquisition.

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Correspondence to Wei Gong or Jing Li.

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Li, LS., Liang, HW., Wu, CT. et al. A ratiometric fluorescence sensor for tetracycline detection based on two fluorophores derived from Partridge tea. Microchim Acta 190, 66 (2023). https://doi.org/10.1007/s00604-023-05653-x

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  • DOI: https://doi.org/10.1007/s00604-023-05653-x

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