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Aptasensor-based assay for dual-readout determination of aflatoxin B1 in corn and wheat via an electrostatic force–mediated FRET strategy

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Abstracts

A rapid and sensitive aptasensor was established for the dual-readout determination of aflatoxin B1 (AFB1) utilizing an electrostatically mediated fluorescence resonance energy transfer (FRET) signal amplification strategy. In the presence of AFB1, the aptamer preferentially bound to AFB1, resulting in the aggregation of bare gold nanoparticles (AuNPs) induced by NaCl, accompanied by a change of AuNP solution from wine-red to purple. This color change was used for colorimetric channel analysis. Then, the positively charged quantum dots were introduced into reaction system and interacted with negatively charged AuNPs, which successfully converted the color signal into a more sensitive fluorescence signal through FRET. The fluorescence quenching efficiency decreased with increasing concentrations of AFB1, and the fluorescence of aptasensor gradually recovered. The variation of fluorescence intensity was employed for fluorometric channel analysis. Under the optimal conditions, the color and fluorescence signals exhibited excellent response to AFB1 concentration within the ranges 10–320 ng·mL−1 and 3–320 ng·mL−1, respectively, and the limit of detection was as low as 7.32 ng·mL−1 and 1.48 ng·mL−1, respectively. The proposed aptasensor exhibited favorable selectivity, good recovery (85.3–113.4% in spiked corn and wheat samples), stable reproducibility (RSD<13.3%), and satisfactory correlation with commercial kits (R2=0.998). The aptasensor developed integrates advantages of modification-free, dual-readout, self-calibration, easy operation, and cost-effectiveness, while providing a simple and universal strategy for rapid and sensitive detection of mycotoxins in foodstuffs.

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Funding

We gratefully acknowledge the support of Beijing Natural Science Foundation of China (Grant No. 6222021).

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Authors and Affiliations

  1. National Key Laboratory of Veterinary Public Health Security, College of Veterinary Medicine, China Agricultural University, Beijing, 100193, China

    Jincheng Xiong, Shuang He, Linqian Qin, Shuai Zhang, Wenchong Shan & Haiyang Jiang

  2. Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety, China Agricultural University, Beijing, 100193, China

    Jincheng Xiong, Shuang He, Linqian Qin, Shuai Zhang, Wenchong Shan & Haiyang Jiang

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  1. Jincheng Xiong
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Contributions

Jincheng Xiong: conceptualization, data curation, formal analysis, writing—original draft. Shuang He: investigation, methodology, data analysis. Linqian Qin: investigation, methodology. Shuai Zhang: methodology, data curation. Wenchong Shan: investigation, data curation, formal analysis. Haiyang Jiang: resources; supervision; writing—review and editing; funding acquisition; project administration.

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Correspondence to Haiyang Jiang.

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Xiong, J., He, S., Qin, L. et al. Aptasensor-based assay for dual-readout determination of aflatoxin B1 in corn and wheat via an electrostatic force–mediated FRET strategy. Microchim Acta 190, 80 (2023). https://doi.org/10.1007/s00604-023-05641-1

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