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Biomimetic nanochannels for molybdate transport: application to sensitive electrochemical immunoassay for HER2

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Abstract

A nanochannel-based electrochemical immunoassay was developed for the detection of human epidermal growth factor receptor 2 (HER2), with molybdate as the reporter to explore the interaction occurring into the nanochannels. The presence of target increased steric hindrance of the antibody-functionalized nanochannels, thereby hindering the transport of molybdate. And the reporter could be monitored by working electrode modified with hydroxyapatite nanoparticles, based on the formation of the redox-active molybdophosphate. As a result, peak current obtained at ca. − 0.28 V in square wave voltammograms could be applied to quantitative determination of HER2. The electrochemical signal increased linearly with the logarithm of the concentration of HER2 in a broad dynamic range of 0.1 pg∙mL−1 to 10 ng∙mL−1 with a detection limit of 0.05 pg∙mL−1. The reliability of this immunoassay was validated by a recovery range of 99.5% to 111.7% for the detection of three different levels of HER2 in human serum samples. Integrating with multiple bionanochannels, this immunoassay is expected to provide a versatile approach for quantitative detection of various biomarkers in related disease diagnosis and therapy.

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Funding

This study was financially supported by the National Natural Science Foundation of China (No. 21775120).

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Authors and Affiliations

  1. Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of the Ministry of Education, College of Chemistry & Materials Science, Northwest University, Xi’an, 710127, Shaanxi, China

    Chunyang Du, Jiao Jiao & Hongfang Zhang

  2. Department of Applied Chemistry, Yuncheng University, Yuncheng, 044000, Shanxi, China

    Jiao Jiao

Authors
  1. Chunyang Du
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  2. Jiao Jiao
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  3. Hongfang Zhang
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Correspondence to Hongfang Zhang.

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Du, C., Jiao, J. & Zhang, H. Biomimetic nanochannels for molybdate transport: application to sensitive electrochemical immunoassay for HER2. Microchim Acta 190, 53 (2023). https://doi.org/10.1007/s00604-023-05632-2

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