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Photothermal biosensor for HPV16 based on strand-displacement amplification and gold nanoparticles using a thermometer as readout

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Abstract

Gold nanoparticles (AuNPs) in aggregated state have a strong near infrared region (NIR) absorption and the causes a much stronger photothermal effect than that of the dispersed AuNPs. Strand-displacement amplification (SDA) can produce large amount of single-stranded DNA (ssDNA), which in turn effectively prevent AuNPs from aggregation. In this study, these characteristics had been applied to design a photothermal biosensor for human papilloma virus (HPV and HPV16 were chosen as model target) detection. In the absence of HPV16, AuNPs was in the aggregated state and large temperature rise can be detected after the irradiation by 808 nm laser. The presence of HPV16 triggers the SDA reaction with the help of Bst DNA polymerase and Nt.BstNBI nicking endonuclease resulting in the production of large amounts of ssDNA; this protects unmodified AuNPs from salt-induced aggregation. Therefore, AuNPs was in a dispersed state and the temperature change was not significant after the irradiation of 808 nm laser. The difference of the temperature changing can be applied for the quantitative detection of HPV16 using a thermometer as readout. The linear response range is 1.0 fM ~ 50 pM with a detection limit of 0.3 fM. The proposed method has been applied to detect HPV16 in clinical cervical sample and is competent for clinical analysis.

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Funding

This project was supported by the National Sciences Foundation of China (21904020, 21974020), Health Science and Technology Project of Zhejiang Province (2022KY887).

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Author notes

  1. Bingyan Yan and Min Li contributed equally to this work

Authors and Affiliations

  1. Department of Infectious Diseases, The Key Laboratory of Diagnosis and Controlment for The Development of Chronic Liver Disease of Zhejiang Province, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325025, China

    Bingyan Yan & XiaoYa Jin

  2. Ministry of Education Key Laboratory for Analytical Science of Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, College of Chemistry, Fuzhou University, 2 Xue Yuan Road, University Town Fuzhou, Fuzhou, 350116, Fujian, People’s Republic of China

    Bingyan Yan, Fang Luo, Bin Qiu & Zhenyu Lin

  3. Department of General Medicine, Fujian Provincial Hospital, Provincial Clinical 13 Medical College of Fujian Medical University, Fuzhou, Fujian, 350001, China

    Min Li

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  1. Bingyan Yan
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Contributions

The manuscript was written through contributions of all authors. B.Y., M.L., and Z.L. conceived the projects, and B.Y., M.L., F.L., X.J., and Z.L. designed and performed the experiments and collected the data. B.Y., M.L., F.L., X.J., B.Q., and Z.L. analyzed and discussed the data. All authors discussed the results and contributed to the writing of this manuscript. All authors have given approval to the final version of the manuscript.

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Correspondence to XiaoYa Jin or Zhenyu Lin.

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Yan, B., Li, M., Luo, F. et al. Photothermal biosensor for HPV16 based on strand-displacement amplification and gold nanoparticles using a thermometer as readout. Microchim Acta 189, 437 (2022). https://doi.org/10.1007/s00604-022-05522-z

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