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Screening of dopamine in living cells and animal model via graphene quantum dots anchored 3D macroporous nonenzymatic sensor

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Abstract

A series of three-dimensional copper oxide (CuO) inverse opals anchored with carboxylated graphene quantum dots (CuO/cGQDs) have been fabricated for non-enzymatic tracking of dopamine (DA). Heterostructures composed of various building blocks are promising to construct versatile biosensing platforms. The optimal CuO/cGQDs modified electrode demonstrates sensitivities of 243.45 μA mM−1 cm−2 (50 nM–1888.5 μM) with the practical detection limit as low as 0.5 nM in mimic physiological environment (at + 0.45 V vs. Ag/AgCl). The extraordinary tolerance to various interferents enables the practical detection of intracellular DA amount in human neural cells. On this basis, the proposed biosensor attains precise evaluation of antipsychotic drug effects on stimulated DA release. Particularly, it successfully spots fluctuation of DA in plasma and cerebrospinal fluid in murine model of Parkinson’s disease, which serves as a crucial tool to understand neuropathology and symptomatology of DA-related diseases. This study developed a reliable sensing platform and is expected to be applied to physiological and pathological studies.

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Funding

This work was supported by the National Science Foundation of China (82170998 and 61874049), General program of Natural Science Foundation of Jilin Province (20200201356JC, 20200201317JC, 20180101210JC and 20200801017GH), seed grant from Jilin University School of Dentistry, and the Fundamental Research Funds for the Central Universities.

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Authors and Affiliations

  1. Department of Oral Implantology, School and Hospital of Stomatology, Jilin University, Changchun, 130021, China

    Kun Wang, Yige Li, Manlin Qi, Chunyan Li & Lin Wang

  2. State Key Laboratory On Integrated Optoelectronics, Collage of Electronic Science and Engineering, Jilin University, Changchun, 130021, China

    Biao Dong & Lin Xu

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  1. Kun Wang
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  2. Yige Li
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  3. Manlin Qi
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  4. Chunyan Li
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  5. Biao Dong
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  6. Lin Xu
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  7. Lin Wang
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Correspondence to Biao Dong, Lin Xu or Lin Wang.

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Wang, K., Li, Y., Qi, M. et al. Screening of dopamine in living cells and animal model via graphene quantum dots anchored 3D macroporous nonenzymatic sensor. Microchim Acta 189, 382 (2022). https://doi.org/10.1007/s00604-022-05479-z

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