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Site-directed dual bioprobes inducing single-step nano-sandwich assay for the detection of cardiac troponin I

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Abstract

Bioreceptor functionalized metallic nano-colloids have been identified as effective nanobioprobes to realize the detection of an analyte based on a common phenomenon of salt-induced aggregation. In marked contrast to this, we describe a nano-sandwich assay integrating the novel match-pair of aptamer and peptide functionalized gold nanoparticles. The site-directed biomolecular interaction of high affinity aptamer and peptide bioreceptors directed towards distinct sites of cardiac biomarker troponin I; this was found to form a nano-sandwich assay in a peculiar manner. The gold nanoconjugates interact with specific and distant regions of troponin I to result in collision of probes upon target identification. In the presence of TnI, both nanobioprobes bind at their respective sites forming a nano-sandwich pair providing a visual color change from red to blue. Thus, the presence of target TnI itself causes instant agglomeration in just a single-step without addition of any external aggregator. The assay imparts 100% specificity and 90% sensitivity in a dynamic concentration range of 0.1–500 ng/mL troponin I with detection limit as low as 0.084 ng/mL. The applicability of the assay has been validated in clinical samples of acute myocardial infarction patients thus establishing a promising point-of-care detection of TnI.

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Acknowledgements

The authors want to thank DST-INSPIRE and ICMR-SRF for providing fellowships to SK and B. The authors also acknowledge Mr Randeep Sharma for TEM experiments.

Funding

The work done in the study has been supported by CSIR-Mission project under project HCP-012.

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Author notes

  1. Saloni Kakkar and Sakshi Chauhan contributed equally to this work.

Authors and Affiliations

  1. Institute of Microbial Technology (IMTECH), Council of Scientific and Industrial Research (CSIR), Chandigarh, India, 160036

    Saloni Kakkar, Sakshi Chauhan, Rajni Bala,  Bharti, Virendra Kumar & Vijayender Bhalla

  2. Department of Cardiology, PGIMER, Chandigarh, India, 160012

    Manojkumar Rohit

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  1. Saloni Kakkar
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Kakkar, S., Chauhan, S., Bala, R. et al. Site-directed dual bioprobes inducing single-step nano-sandwich assay for the detection of cardiac troponin I. Microchim Acta 189, 366 (2022). https://doi.org/10.1007/s00604-022-05461-9

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