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A signal-off photoelectrochemical aptasensor for ultrasensitive 17β-estradiol detection based on rose-like CdS@C nanostructure and enzymatic amplification

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Abstract

Carbon-coated cadmium sulfide rose-like nanostructures (CdS@C NRs) were prepared via a facile solvothermal approach and used as the photoelectrochemical (PEC) sensing platform for the integration of functional biomolecules. Based on this, a novel “signal-off” PEC aptasensor mediated by enzymatic amplification was proposed for the sensitive and selective detection of 17β-estradiol (E2). In the presence of E2, alkaline phosphatase-modified aptamer (ALP-apta) were released from the electrode surface through the specific recognition with E2, which caused the negative effect on PEC response due to the decrease of ascorbic acid (AA) produced by the ALP in situ enzymatic catalysis. The developed PEC aptasensor for detection of E2 exhibited a wide linear range of 1.0–250 nM, with the low detection limit of 0.37 nM. This work provides novel insight into the design of potential phoelectroactive materials and the application of signal amplification strategy in environmental analysis field.

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Funding

This research was supported by Natural Science Foundation of Zhejiang Province (LQ20B050001), Jinhua Science and Technology Bureau (No. 2020–4-187, 2021–3-058), and Zhejiang Public Welfare Technology Application Research Project (LGG19B050001).

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  1. Lian-Hong Tu and Jian-Hong Zhu contributed equally to this work.

Authors and Affiliations

  1. College of Geography and Environmental Sciences, College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua, 321004, China

    Lian-Hong Tu, Jian-Hong Zhu, Aisyah-protonia Tanjung, Min Wang, Jinwei Kang, Ai-Jun Wang & Li-Ping Mei

  2. Central Laboratory, Affiliated Jinhua Hospital, Zhejiang University School of Medicine, Jinhua, 321000, China

    Yadong Xue & Pei Song

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  1. Lian-Hong Tu
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Tu, LH., Zhu, JH., Tanjung, Ap. et al. A signal-off photoelectrochemical aptasensor for ultrasensitive 17β-estradiol detection based on rose-like CdS@C nanostructure and enzymatic amplification. Microchim Acta 189, 56 (2022). https://doi.org/10.1007/s00604-022-05164-1

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