Abstract
An immunoassay strategy has been developed based on nanomotor-assisted electrochemical measurements for simple and sensitive detection of immunoglobulin (IgG). The self-propelled Fe3O4@SiO2/Pt nanomotors were designed to label primary antibodies IgG (nanomotor-label) for the “on-the-fly” binding of the immune-protein. The core shell Au@Ag nanocubes (Au@Ag NCs) were used as labels of secondary antibodies (Au@Ag NCs-Ab2) to amplify electrochemical signal related to antigen concentration derived from the oxidation of Ag. The self-propelled nanomotors autonomously move in the solution to cruise and capture IgG and Au@Ag NCs-Ab2, resulting in the self-assembly of sandwich immune-complex. Finally, the immune-complex with magnetism can be transferred and modified on the electrode for the detection of IgG via differential pulse voltammetry. The self-propelled motion of the nanomotor-label obviates common procedures for the self-assembly of sandwich immunosensors to achieve satisfactory analysis results. With advantages of automation and miniaturization, the strategy based on self-propelled nanomotor-labels explores an effective method for the simple and sensitive detection of immune-protein in biosensing.
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This work is supported by the National Natural Science Foundation of China (21905303) and the Fundamental Research Funds for the Central Universities (2020ZDPY0213).
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Ma, E., Wang, K. & Wang, H. An immunoassay based on nanomotor-assisted electrochemical response for the detection of immunoglobulin. Microchim Acta 189, 47 (2022). https://doi.org/10.1007/s00604-021-05158-5
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DOI: https://doi.org/10.1007/s00604-021-05158-5