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Specific determination of HBV using a viral aptamer molecular imprinting polymer sensor based on ratiometric metal organic framework

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Abstract

An approach is reported based on the combination of aptamer and metal organic frameworks (MOF) to prepare a molecularly imprinted sensor that recognizes viruses with high specificity and sensitivity. Using MIL-101-NH2 as a polymer carrier, viral aptamers were introduced into the carrier surface through an amide reaction to specifically identify the target, and surface imprinting is carried out through tetraethyl silicate (TEOS) self-polymerization. The MIL-101-NH2 is also used as the reference fluorescence signal (λex/λem = 290/460 nm) and rhodamine B as the change signal (λex/λem = 550/570 nm). The ratiometric fluorescence detection and dual recognition strategy not only reduce environmental interference but also greatly improve the sensor’s anti-interference ability, the obtained imprinting factor was 5.72, and the detection limit as low as 1.8 pmol L−1. Therefore, the molecular imprinting sensor designed realizes the specific and highly sensitive identification of viruses, which provides theoretical support for the application of molecular imprinting technology in clinical diagnosis of viruses.

Aptamer-molecular imprinting polymer based on metal-organic framework ratiometric fluorescent detect virus.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (No. 21775132), the National Natural Science Foundation of Hunan province (No. 2018JJ2388), Hunan 2011 Collaborative Innovation Center of Chemical Engineering & Technology with Environmental Benignity and Effective Resource Utilization, the project of the innovation team of the ministry of education (IRT_17R90).

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Authors and Affiliations

  1. Foshan Green Intelligent Manufacturing Research Institute of Xiangtan University, Key Laboratory for Green Organic Synthesis and Application of Hunan Province, Key Laboratory of Environmentally Friendly Chemistry and Application of Ministry of Education, College of Chemistry, Xiangtan University, Xiangtan, 411105, China

    Lingyun Wang, Junyu Yang, Li Tang, Lianghui Luo, Chunyan Chen, Hang Gong & Changqun Cai

  2. School of Material and Chemical Engineering, Hunan Institute of Technology, Hengyang, 421002, China

    Lingyun Wang

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  1. Lingyun Wang
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  3. Li Tang
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Contributions

LW: methodology, conceptualization, writing—review and editing. JY: conceptualization, writing—review and editing. LT: methodology, validation, software, and editing. LL: methodology, conceptualization, software. CC: validation, data curation. HG: formal analysis, writing—review and editing. CC: writing–review and editing, supervision, project administration, funding acquisition.

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Correspondence to Chunyan Chen or Changqun Cai.

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Supplementary Information

The Supporting Information is available free of charge on the ACS Publications website. Additional details of structure of XRD (Fig. S1), TGA (Fig. S2), N2 adsorption-desorption isotherm of (A) MIL-101-NH2 and (B) MIPs (Fig. S3). The influence of the amount of aptamer on IF and (F590/F460)0/(F590/F460) of MIPs and The influence of imprinting thickness on IF and (F590/F460)0/(F590/F460) of MIPs (Fig. S4). Optimization of the assay conditions (Fig. S5 - Fig. S9). Linearity of H-MIPs (Fig. S10), Fluorescence lifetime and UV–vis absorption spectrum of different species about MIP (Fig. S11), IF and SF (Table S1).

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Wang, L., Yang, J., Tang, L. et al. Specific determination of HBV using a viral aptamer molecular imprinting polymer sensor based on ratiometric metal organic framework. Microchim Acta 188, 221 (2021). https://doi.org/10.1007/s00604-021-04858-2

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