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An impedimetric immunosensor for determination of porcine epidemic diarrhea virus based on the nanocomposite consisting of molybdenum disulfide/reduced graphene oxide decorated with gold nanoparticles

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Abstract

An electrochemical immunosensor for the determination of porcine epidemic diarrhea virus (PEDV) is described. It was manufactured by using gold nanoparticles/molybdenum disulfide/reduced graphene oxide nanocomposites modified on the surface of a glassy carbon electrode (GCE). The independently developed monoclonal antibody of PEDV-2C11 was immobilized on the modified electrode at site of gold nanoparticles provided in the nanocomposites. The concentration of PEDV was quantified by measuring the changes in the charge transfer resistance of the electrode before and after the immunoreaction between antigen-antibody by using hexacyanoferrate(II)/(III) as the redox probe. The frequency range was 10−1 to 105 Hz at the amplitude of 10 mV and an applied potential of + 0.180 V. Based on the immunoreaction between PEDV antigen and PEDV-2C11 antibody in 0.1 M phosphate buffer containing 0.1 M KCl at 37.5 °C for 140 min, the relative change in impedance was proportional to the logarithmic value of PEDV concentrations in the range of 82.5 to 1.65 × 104 TCID50 mL−1. Good reproducibility, stability, and specificity of the proposed immunosensor were obtained. It was successfully applied to the determination of PEDV in the spiked sample.

Schematic representation. a The preparation of AuNP/MoS2/rGO composites. b Representation of modification and functioning of the label-free electrochemical immunosensor and the electrochemical impedimetric response obtained before (a) and after (b) incubation of PEDV

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Acknowledgments

The authors are thankful to Prof. Ming Shen, School of Chemistry and Chemical Engineering, Yangzhou University, for assistance in material synthesis.

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  1. Xiaotong Li and Yanchun Wang contributed equally to this work.

Authors and Affiliations

  1. Key Laboratory of Drug Quality Control and Pharmacovigilance (China Pharmaceutical University), Ministry of Education, School of Pharmacy, China Pharmaceutical University, 210009, Nanjing, People’s Republic of China

    Xiaotong Li, Xiaolei Zhang, Ye Gao, Fang Feng & Gongjun Yang

  2. College of Veterinary Medicine, Ministry of Education key laboratory of poultry preventive medicine, Yangzhou University, 225002, Yangzhou, People’s Republic of China

    Yanchun Wang & Wenjie Jin

  3. College of Chemistry and Chemical Engineering, Yangzhou University, 225002, Yangzhou, People’s Republic of China

    Chenmin Sun & Yuanhua Ding

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  1. Xiaotong Li
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Correspondence to Fang Feng, Wenjie Jin or Gongjun Yang.

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Li, X., Wang, Y., Zhang, X. et al. An impedimetric immunosensor for determination of porcine epidemic diarrhea virus based on the nanocomposite consisting of molybdenum disulfide/reduced graphene oxide decorated with gold nanoparticles. Microchim Acta 187, 217 (2020). https://doi.org/10.1007/s00604-020-4166-2

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