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A colorimetric immunoassay for determination of Escherichia coli O157:H7 based on oxidase-like activity of cobalt-based zeolitic imidazolate framework

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Abstract

Cobalt-based zeolitic imidazolate framework nanosheets (ZIF-67) with oxidase-like catalytic activities as an immunoprobe were employed to enhance the sensitivity of an immunoassay. ZIF-67 was synthesized via the solvothermal method using 2-methylimidazole and cobalt dichloride as substrates. A colorimetric immunoassay for Escherichia coli (E. coli) O157:H7 was designed. Preparation of the immunoprobe involved self-polymerized dopamine being applied for the surface modification of ZIF-67 nanosheets in order to bind to the antibody, which was used to identify E. coli O157:H7. ZIF-67 catalyze the oxidation of 3,3′,5,5′-tetramethylbiphenyl (TMB) and produced a color change from colorless to blue. Upon reaction termination, the absorbance was measured at 450 nm. By combining ZIF-67@PDA catalyzed chromogenic reaction with antibody recognition and magnetic separation, the limit of determination is 12 CFU mL−1 and the linear range is 30 to 3.0 × 108 CFU mL−1. The proposed colorimetric immunoassay was successfully utilized to detect E. coli O157:H7 of spiked food samples.

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Funding

We acknowledge the financial support of the National Key R&D Program of China (2018YFC1602500), the Science and Technology Innovation Plan of Shanghai (No. 18495800400), the National Natural Science Foundation of China (No. 81572809, 31871897), and the National Natural Science Foundation for Young Scientists of China (No. 81502504, 31801455).

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Authors and Affiliations

  1. School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai, 200093, China

    Shujuan Wang, Dongpo Xu, Chengchao Ding, Yachen Tian, Kangjie Ge, Liang Guo, Jie Li, Qingli Dong & Qing Liu

  2. Affiliated Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu University of Traditional Chinese Medicine, Sichuan, 611137, Chengdu, China

    Yong Huang

  3. Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266071, People’s Republic of China

    Qing Liu

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  1. Shujuan Wang
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Wang, S., Xu, D., Ding, C. et al. A colorimetric immunoassay for determination of Escherichia coli O157:H7 based on oxidase-like activity of cobalt-based zeolitic imidazolate framework. Microchim Acta 187, 506 (2020). https://doi.org/10.1007/s00604-020-04407-3

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