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Hemin@carbon dot hybrid nanozymes with peroxidase mimicking properties for dual (colorimetric and fluorometric) sensing of hydrogen peroxide, glucose and xanthine

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Abstract

The multifunctional hemin@carbon dot hybrid nanozymes (hemin@CD) with simultaneous peroxidase-like activity and fluorescence signalling property was prepared for the first time. Based on these properties, hemin@CD was applied to develop a dual-channel fluorescent probe for H2O2 and H2O2-based biocatalytic systems. By virtue of the peroxidase-like activity, hemin@CD can catalyze the oxidative coupling of 4-aminoantipyrine with phenol in the presence of H2O2 to form a pink-red quinoneimine dye with a maximum absorbance at 505 nm. Under the excitation wavelength of 480 nm, the green fluorescence of hemin@CD peaks at 540 nm and is quenched by the generated quinoneimine dye due to an inner filter effect, and also by H2O2 because of dynamic quenching. Thus, a colorimetric and fluorimetric dual-channel optical probe for H2O2 is obtained. Due to the glucose/xanthine transformations under formation of H2O2 by the relevant oxidase catalysis, the probe can be applied for detection of glucose and xanthine. The colorimetric detection limits for H2O2, glucose and xanthine are 0.11, 0.15, 0.11 μM, and the and fluorimetric detection limits are 0.15, 0.15, 0.12 μM, respectively.

Schematic representation of the colorimetric and fluorimetric dual probe for H2O2, glucose and xanthine based on the multifunctional emin@carbon dot) hybrid nanozymes with simultaneous peroxidase-like activity and fluorescence signalling property.

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Acknowledgments

This work was financially supported by National Natural Science Foundation of China (21405034, 21722501, 21976052). Key Project of Science and Technology of Henan Province (192102210041). Program for Science Technology Innovation Talents in Universities of Henan Province (18HASTIT001). Dr. start-up project funding of Henan Normal University (qd18014).

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Authors and Affiliations

  1. Collaborative Innovation Center of Henan Province for Green Manufacturing of Fine Chemicals, Key Laboratory of Green Chemical Media and Reactions, Ministry of Education, School of Chemistry and Chemical Engineering, Henan Normal University, Xinxiang, Henan, 453007, People’s Republic of China

    Li Su, Yexi Cai, Liang Wang, Wenpei Dong, Guojiang Mao, Ye Li, Mingsheng Zhao & Hua Zhang

  2. College of Chemistry and Chemical Engineering, Liaocheng University, Liaocheng, Shandong, 252059, People’s Republic of China

    Yanhua Ma

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  1. Li Su
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Su, L., Cai, Y., Wang, L. et al. Hemin@carbon dot hybrid nanozymes with peroxidase mimicking properties for dual (colorimetric and fluorometric) sensing of hydrogen peroxide, glucose and xanthine. Microchim Acta 187, 132 (2020). https://doi.org/10.1007/s00604-019-4103-4

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