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Electrochemiluminescence based detection of microRNA by applying an amplification strategy and Hg(II)-triggered disassembly of a metal organic frameworks functionalized with ruthenium(II)tris(bipyridine)

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Abstract

An electrochemiluminescence (ECL) biosensor is described for the detection of microRNA (miRNA-155) based on tris(bipyridine)ruthenium(II) functionalized metal organic framework (RuMOF) materials. The material was prepared by a solvothermal method and was found to be stable even in acidic solution. However, it is selectively and sensitively disassembled by Hg(II) ions, resulting in the release of large quantities of Ru(II)(bpy)3 ions, which produces a strong ECL signal. In view of the ion-selective disassembly and release and strand displacement process, an ultrasensitive ECL sensing method was established for detection of microRNAs. In the presence of the target, the hairpin structure of H1 can open and hybridize with the hairpin probe H2 to form a more stable H1-H2 duplex structure than the H1-target hybrid. The target of hybridization to H1 was immediately freed from the structure and the released target re-entered the new hairpin assembly target recovery process. The remaining H2 single fragment can bind to the I-RuMOFs-conjugates. The more hairpin probes H1, the more I-RuMOFs-conjugates load the DNA fragments, leading to the signal amplification. The method works in the 0.8 f. to 1.0 nM miRNA-155 concentration range and has a detection limit of 0.3 fM. The assay is sensitive, fairly specific and remarkably stable. In our perception, it offers an attractive tool for the sensitive detection of microRNAs in clinical samples.

An electrochemiluminescence (ECL) based biosensor is described for the detection of microRNA (miRNA-155) based on the use of a metal organic framework functionalized with ruthenium(II)tris(bipyridine) that was deposited on a glassy carbon electrode (GCE) modified with gold nanoparticles.

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Acknowledgements

This work was financially supported by the National Natural Science Foundation of China (21575051, 21775055, 21501090), 111 Project of International Corporation on Advanced Cement-based Materials (No. D17001) and the program of Taishan Scholars.

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Authors and Affiliations

  1. Institute for Advanced Interdisciplinary Research, University of Jinan, Jinan, 250022, People’s Republic of China

    Yannan Jian, He Wang, Feifei Lan, Linlin Liang, Na Ren, Haiyun Liu, Shenguang Ge & Jinghua Yu

  2. Key Laboratory of Interfacial Reaction & Sensing Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan, 250022, People’s Republic of China

    Yannan Jian & Jinghua Yu

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  1. Yannan Jian
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  2. He Wang
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  3. Feifei Lan
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  6. Haiyun Liu
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Correspondence to Haiyun Liu or Shenguang Ge.

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Jian, Y., Wang, H., Lan, F. et al. Electrochemiluminescence based detection of microRNA by applying an amplification strategy and Hg(II)-triggered disassembly of a metal organic frameworks functionalized with ruthenium(II)tris(bipyridine). Microchim Acta 185, 133 (2018). https://doi.org/10.1007/s00604-018-2693-x

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