Abstract
Blood analysis is a critical and potent diagnostic tool used in the field of fisheries to determine the well-being status of the fish. However, internal and external influences, such as anticoagulants and storage time, can have a significant impact on fish hematological values resulting in species-specific reactions. Because of the short clotting periods, fish blood generally needs the inclusion of an anticoagulant agent; therefore, choosing the right anticoagulant is imperative for achieving accurate blood test results. The aim of current study is to analyze the impact of three different anticoagulants (lithium heparin, di-potassium EDTA, and sodium citrate) on various hematological characteristics of snow trout, Schizothorax labiatus, and their effect on different intervals of storage time at + 4 °C. The results revealed that Hb, Hct, MCH, and MCV in all the samples treated with K2EDTA produced significantly (P < 0.05) higher values than other two anticoagulants. While, total RBC count was recorded significantly (P < 0.05) higher in lithium heparin–treated samples. However, total WBC count and MCHC in all the three anticoagulant-treated samples exhibited insignificant (P > 0.05) difference. After analysis at 0 h, the blood samples were refrigerated at + 4 °C for 24 h, 48 h, and 72 h, respectively. Statistical evaluation revealed significant (P < 0.05) impact of storage time on values of Hb, RBC, Hct, MCH, MCHC, and MCV except WBC after 24 h of time interval. Hence, on the basis of above mentioned findings, it is suggested that for fish blood analysis, lithium heparin provides more reliable data with a higher level of anticoagulant dependability in S. labiatus and also the blood samples must be analyzed within 24 h after collection when stored at + 4 °C as indicated in the present study that longer storage affects the hematological profile of the fish.
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Abbreviations
- EDTA:
-
Ethylene diamine tetra acetate
- Hb:
-
Hemoglobin
- RBC:
-
Red blood cell count
- WBC:
-
White blood cell count
- Hct:
-
Hematocrit
- MCH:
-
Mean corpuscular hemoglobin
- MCHC:
-
Mean corpuscular hemoglobin concentration
- MCV:
-
Mean corpuscular volume
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Acknowledgements
The authors are highly grateful to the Head of the Department of Zoology for providing necessary laboratory facilities and also grateful to the Department of Science and Technology (DST), Govt of India, New Delhi, for providing financial assistance as DST-INSPIRE fellowship to one of the author, namely, Kousar Jan.
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Jan, K., Ahmed, I., Sheikh, Z.A. et al. Impact of three anticoagulants and their storage time on hematological parameters of snow trout, Schizothorax labiatus, habiting in river Sindh of Indian Himalayan region. Comp Clin Pathol 31, 747–755 (2022). https://doi.org/10.1007/s00580-022-03375-9
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DOI: https://doi.org/10.1007/s00580-022-03375-9