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Anti-diabetic and hepato-renal protective potential of hydroalcoholic extract of Teucrium polium in alloxan-induced diabetic rats

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Abstract

Diabetes mellitus is an endocrine disorder and one of the most common metabolic diseases. Teucrium polium has been exerted nephro- and hepatoprotective effects. This study’s objective was to determine the effects of hydroalcoholic extract of Teucrium polium (T. polium) on histopathological and biochemical markers of the liver and kidney in diabetic rats. In this experimental study, 60 male Wistar rats were divided to healthy control (group 1), diabetic control (group 2, these two groups received distilled water), treated diabetic positive control (group 3) using a dose 150 mg/kg/day metformin, and two groups treated with doses 400 (group 4) and 800 (group 5) mg/kg/BW of T. polium extracts via gavage feeding for 8 weeks. Diabetes mellitus was experimentally induced by one dose intraperitoneally injection of alloxan 120 mg/kg. Comparison of normal rats and diabetic rats revealed that kidney tissue would experience tubular changes in diabetic rats. Histopathological examination revealed a reduction in the tubular changes of kidney tissue following treatment with the extract. In addition, the T. polium extract was able to reduce the infiltration of PMN cells in the liver tissue. Also, T. polium significantly decreased blood glucose, malondialdehyde, and creatinine levels. Results suggested that T. polium extract can decrease hepato-renal damages by influencing histopathological and biochemical markers in diabetic rats.

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References

  • Abdallah AA, Nasr El-Deen NA, Abd El-Aziz HI, Neamat-Allah AN (2020) Effect of the aqueous root extract of Curcuma longa L. (turmeric) against thermally oxidized oil-induced hematological, biochemical and histopathological alterations. Comp Clin Pathol 29:837–845

    Article  CAS  Google Scholar 

  • Agarwal R, Chase SD (2002) Rapid, fluorimetric–liquid chromatographic determination of malondialdehyde in biological samples. J Chromatogr B 775:121–126

    Article  CAS  Google Scholar 

  • Akbari F, Ansari Samani R, Karimi A, Mortazaei S, Shahinfard N, Rafieian-Kopaei M (2013) Effect of turnip on glucose and lipid profiles of alloxan-induced diabetic rats. Iranian J Endocrinol Metab 14

  • Alok S, Jain SK, Verma A, Kumar M, Sabharwal M (2013) Pathophysiology of kidney, gallbladder and urinary stones treatment with herbal and allopathic medicine: a review. Asian Pac J Trop Dis 3:496–504

    Article  Google Scholar 

  • Ardestani A, Yazdanparast R, Jamshidi S (2008) Therapeutic effects of Teucrium polium extract on oxidative stress in pancreas of streptozotocin-induced diabetic rats. J Med Food 11:525–532

    Article  CAS  Google Scholar 

  • Carleton H, Drury R, Wallington E (1980) Carleton’s histological technique. Oxford University Press, USA

    Google Scholar 

  • Darabpour E, Motamedi H, Nejad SMS (2010) Antimicrobial properties of Teucrium polium against some clinical pathogens. Asian Pac J Trop Med 3:124–127

    Article  Google Scholar 

  • El-Haskoury R, Al-Waili N, El-Hilaly J, Al-Waili W, Lyoussi B (2019) Antioxidant, hypoglycemic, and hepatoprotective effect of aqueous and ethyl acetate extract of carob honey in streptozotocin-induced diabetic rats. Veterinary World 12:1916

    Article  CAS  Google Scholar 

  • Esmaeili MA, Yazdanparast R (2004) Hypoglycaemic effect of Teucrium polium: studies with rat pancreatic islets. J Ethnopharmacol 95:27–30

    Article  Google Scholar 

  • Gao C, Sun X, Lu L, Liu F, Yuan J (2019) Prevalence of gestational diabetes mellitus in mainland China: a systematic review and meta-analysis. J Diabetes Investig 10:154–162

    Article  CAS  Google Scholar 

  • Grankvist K, Marklund S, Täljedal I-B (1981) Superoxide dismutase is a prophylactic against alloxan diabetes. Nature 294:158–160

    Article  CAS  Google Scholar 

  • Hashem MA, Neamat-Allah AN, Hammza HE, Abou-Elnaga HM (2020) Impact of dietary supplementation with Echinacea purpurea on growth performance, immunological, biochemical, and pathological findings in broiler chickens infected by pathogenic E. coli. Trop Anim Health Prod 52:1599–1607

    Article  Google Scholar 

  • Hughes TA, Gwynne JT, Switzer BR, Herbst C, White G (1984) Effects of caloric restriction and weight loss on glycemic control, insulin release and resistance, and atherosclerotic risk in obese patients with type II diabetes mellitus. Am J Med 77:7–17

    Article  CAS  Google Scholar 

  • Jami M-S, Pal R, Hoedt E, Neubert TA, Larsen JP, Møller SG (2014) Proteome analysis reveals roles of L-DOPA in response to oxidative stress in neurons. BMC Neurosci 15:1–11

    Article  Google Scholar 

  • Jami M-S, Salehi-Najafabadi Z, Ahmadinejad F, Hoedt E, Chaleshtori MH, Ghatrehsamani M, Neubert TA, Larsen JP, Møller SG (2015) Edaravone leads to proteome changes indicative of neuronal cell protection in response to oxidative stress. Neurochem Int 90:134–141

    Article  CAS  Google Scholar 

  • Jelodar G, Nazifi S (1999) Effect of coriander, pomegranate and walnut leaf on serum biochemical parameters of diabetic rats. J Shahid Sadoughi Univer Med Sci 7:77–85

    Google Scholar 

  • Knowler WC, Barrett-Connor E, Fowler SE, Hamman RF, Lachin JM, Walker EA, Nathan DM (2002) Reduction in the incidence of type 2 diabetes with lifestyle intervention or metformin. N Engl J Med 346:393–403

    Article  CAS  Google Scholar 

  • Mahmoud EA, El-Sayed BM, Mahsoub YH, Neamat-Allah AN (2020) Effect of Chlorella vulgaris enriched diet on growth performance, hemato-immunological responses, antioxidant and transcriptomics profile disorders caused by deltamethrin toxicity in Nile tilapia (Oreochromis niloticus). Fish Shellfish Immunol 102:422–429

    Article  CAS  Google Scholar 

  • Mazokopakis E, Lazaridou S, Tzardi M, Mixaki J, Diamantis I, Ganotakis E (2004) Acute cholestatic hepatitis caused by Teucrium polium L. Phytomedicine 11:83–84

    Article  CAS  Google Scholar 

  • Montezano AC, Dulak-Lis M, Tsiropoulou S, Harvey A, Briones AM, Touyz RM (2015) Oxidative stress and human hypertension: vascular mechanisms, biomarkers, and novel therapies. Can J Cardiol 31:631–641

    Article  Google Scholar 

  • Moure A, Franco D, Sineiro J, Domínguez H, Núñez MJ, Lema JM (2000) Evaluation of extracts from Gevuina avellana hulls as antioxidants. J Agric Food Chem 48:3890–3897

    Article  CAS  Google Scholar 

  • Mukhtar Y, Galalain A, Yunusa U (2020) A modern overview on diabetes mellitus: a chronic endocrine disorder. Eur J Biol 5:1–14

    Article  Google Scholar 

  • Nazari A, Fanaei H, Dehpour AR, Hassanzadeh GRE, Jafari M, Salehi M, Mohammadi M (2015) Chemical composition and hepatoprotective activity of ethanolic root extract of Taraxacum syriacum Boiss against acetaminophen intoxication in rats. Bratisl Lek Listy 116:41–46

    CAS  PubMed  Google Scholar 

  • Neamat-Allah AN, Mahmoud EA, Mahsoub Y (2021a) Effects of dietary white mulberry leaves on hemato-biochemical alterations, immunosuppression and oxidative stress induced by Aeromonas hydrophila in Oreochromis niloticus. Fish Shellfish Immunol 108:147–156

    Article  CAS  Google Scholar 

  • Neamat-Allah AN, Mahsoub YH, Mahmoud EA (2021b) The potential benefits of dietary β-glucan against growth retardation, immunosuppression, oxidative stress and expression of related genes and susceptibility to Aeromonas hydrophila challenge in Oreochromis niloticus induced by herbicide pendimethalin. Aquac Res 52:518–528

    Article  CAS  Google Scholar 

  • Otoom S, Al-Safi S, Kerem Z, Alkofahi A (2006) The use of medicinal herbs by diabetic Jordanian patients. J Herb Pharmacother 6:31–41

    Article  CAS  Google Scholar 

  • Polymeros D, Kamberoglou D, Tzias V (2002) Acute cholestatic hepatitis caused by Teucrium polium (golden germander) with transient appearance of antimitochondrial antibody. J Clin Gastroenterol 34:100–101

    Article  Google Scholar 

  • Pourmorad F, Hosseinimehr S, Shahabimajd N (2006) Antioxidant activity, phenol and flavonoid contents of some selected Iranian medicinal plants. Afri J Biotechnol 5

  • Rafieian-Kopaei M, Nasri H (2014) The ameliorative effect of Zingiber officinale in diabetic nephropathy. Iran Red Crescent Med J 16

  • Rahimi-Madiseh M, Heidarian E, Kheiri S, Rafieian-Kopaei M (2017a) Effect of hydroalcoholic Allium ampeloprasum extract on oxidative stress, diabetes mellitus and dyslipidemia in alloxan-induced diabetic rats. Biomed Pharmacother 86:363–367

    Article  CAS  Google Scholar 

  • Rahimi-Madiseh M, Karimian P, Kafeshani M, Rafieian-Kopaei M (2017b) The effects of ethanol extract of Berberis vulgaris fruit on histopathological changes and biochemical markers of the liver damage in diabetic rats. Iran J Basic Med Sci 20:552

    PubMed  PubMed Central  Google Scholar 

  • Rahimi-Madiseh M, Naimi A, Heydarian E, Rafieian-Kopaei M (2017c) Renal biochemical and histopathological alterations of diabetic rats under treatment with hydro alcoholic Morus nigra extract. J Renal Inj Prev 6:56

    Article  CAS  Google Scholar 

  • Rahimi Madiseh M, Heidarian E, Rafieian-Kopaei M (2014) Biochemical components of Berberis lycium fruit and its effects on lipid profile in diabetic rats. J HerbMed Pharmacol 3

  • Rajabalian S (2008) Methanolic extract of Teucrium polium L potentiates the cytotoxic and apoptotic effects of anticancer drugs of vincristine, vinblastine and doxorubicin against a panel of cancerous cell lines. Exp Oncol

  • Rasekh H, Khoshnood-Mansourkhani M, Kamalinejad M (2001) Hypolipidemic effects of Teucrium polium in rats. Fitoterapia 72:937–939

    Article  CAS  Google Scholar 

  • Rashki KM, Gol A, Dabiri S, Javadi A (2011) Preventive and therapeutic role of garlic (Allium sativum) on renal complications in rats with diabetes mellitus

  • Roche EF, Menon A, Gill D, Hoey H (2005) Clinical presentation of type 1 diabetes. Pediatr Diabetes 6:75–78

    Article  Google Scholar 

  • Roghani M, Aghaie M (2007) The effect Allium ampeloprasum feeding on serum level of glucose, triglyceride, and total cholesterol of diabetic rats. Koomesh 8:73–78

    Google Scholar 

  • Sarraf M, Beig Babaei A, Naji-Tabasi S (2019) Investigating functional properties of barberry species: an overview. J Sci Food Agric 99:5255–5269

    Article  CAS  Google Scholar 

  • Seeff LB, Lindsay KL, Bacon BR, Kresina TF, Hoofnagle JH (2001) Complementary and alternative medicine in chronic liver disease. Hepatology 34:595–603

    Article  CAS  Google Scholar 

  • Selvan V, Manikandan L, Senthil Kumar G, Suresh R, Kakoti B, Gomathi P, Kumar D, Saha P, Gupta M, Mazumder U (2008) Antidiabetic and antioxidant effect of methanol extract of Artanema sesamoides in streptozotocin-induced diabetic rats. Int J Appl Res Nat Prod 1:25–33

    Google Scholar 

  • Shahraki MR, Arab MR, Mirimokadam E, Palan MJ (2007) The effect of Teucrium polium (Calpoureh) on liver function, serum lipids and glucose in diabetic male rats

  • Shakhanbeh J, Adigüzel O (2001) Teucrium polium inhibits nerve conduction and carrageenan induced inflammation in the rat skin. Turk J Med Sci 31:15–21

    Google Scholar 

  • Sharafati-chaleshtori R, Sharafati-chaleshtori F, Rafieian-kopaei M, Ashrafi K (2011) Ethanolic walnut kernel phenolic compounds and its antimicrobial effect. SSU_Journals 19: 525–532

  • Shaw JE, Sicree RA, Zimmet PZ (2010) Global estimates of the prevalence of diabetes for 2010 and 2030. Diabetes Res Clin Pract 87:4–14

    Article  CAS  Google Scholar 

  • Yazdan PR, Esmaeili MA, Ashrafi HJ (2005) Teucrium polium extract effects pancreatic function of streptozotocin diabetic rats: a histopathological examination

  • Zal F, Rasti M, Vesal M, Vaseei M (2001) Hepatotoxicity associated with hypoglycemic effects of Teucrium polium in diabetic rats

  • Zhang X, Tan B (2000) Effects of an ethanolic extract of Gynura procumbens on serum glucose, cholesterol and triglyceride levels in normal and streptozotocin-induced diabetic rats. Singapore Med J 41:9–13

    CAS  PubMed  Google Scholar 

  • Zhou G, Myers R, Li Y, Chen Y, Shen X, Fenyk-Melody J, Wu M, Ventre J, Doebber T, Fujii N (2001) Role of AMP-activated protein kinase in mechanism of metformin action. J Clin Investig 108:1167–1174

    Article  CAS  Google Scholar 

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Funding

This study was funded by Shahrekord University of Medical Sciences [grant no. 1976], Shahrekord, Iran.

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Authors and Affiliations

  1. Department of Biochemistry, School of Medicine, Iran University of Medical Sciences, Tehran, Iran

    Ali Nouri

  2. Medical Plant Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran

    Hossein Amini-Khoei, Mahmoud Rafieian-Kopaei & Mohammad Rahimi-Madiseh

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  1. Ali Nouri
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  2. Hossein Amini-Khoei
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  3. Mahmoud Rafieian-Kopaei
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  4. Mohammad Rahimi-Madiseh
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Contributions

Mohammad Rahimi-Madiseh and Ali Nouri performed experiments and writing the manuscript; Hossein Amini-Khoei analyzed the data and edited the manuscript; Mahmoud Rafieian-Kopaei designed the study.

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Correspondence to Mohammad Rahimi-Madiseh.

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All experimental procedures in this study were approved by the Ethics Committee of Shahrekord University of Medical Sciences, Shahrekord, Iran (Ethic number, IR.SKUMS.REC.1394.150).

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The authors declare no competing interests.

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Nouri, A., Amini-Khoei, H., Rafieian-Kopaei, M. et al. Anti-diabetic and hepato-renal protective potential of hydroalcoholic extract of Teucrium polium in alloxan-induced diabetic rats. Comp Clin Pathol 31, 29–39 (2022). https://doi.org/10.1007/s00580-021-03301-5

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