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Hepatoprotective and anti-inflammatory potential of chebulagic acid on carbon tetrachloride–induced hepatic fibrosis by antioxidative activities in rats

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Abstract

The aim of the present study was to evaluate the hepatoprotective, antioxidant, and anti-inflammatory potential of chebulagic acid on carbon tetrachloride–induced hepatic fibrosis in rats. The liver was damaged in the rats using CCL4 (2 ml/kg b.w dissolved in 20% corn oil) intragastrically twice/week for 8 weeks. After 8 weeks, activities of serum aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase and lactate dehydrogenase, lipid peroxidation markers, and enzymic and non-enzymic antioxidant enzymes, inflammatory and anti-inflammatory cytokines were analyzed and all these parameters were altered. Chebulagic acid administration reversed all the altered parameters to near-normal levels. Improved histological and immunohistochemical observations liver supported the biochemical investigated. Hence, chebulagic acid exhibits hepatoprotective effects against CCl4-induced liver damage.

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Funding

The authors are thankful to the DST-SERB ECR project, New Delhi, India, for providing financial assistance to purchase chebulagic acid for performing this work.

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Authors and Affiliations

  1. Dental Campus, Central Research Laboratory, Meenakshi Academy of Higher Education and Research, Deemed To Be University, West K.K. Nagar, Chennai, Tamil Nadu, 600078, India

    R. Sundaram & V. Ganesh

  2. Department of Biochemistry, Saveetha Dental College & Hospital, Saveetha Institute of Medical & Technical Sciences, Saveetha University, Vellapanchavadi, Chennai, 600077, India

    R. Sundaram

  3. Department of Biochemistry, Sri Muthukumaran Medical College Hospital and Research Institute (Tamil Nadu Dr, MGR Medical University), Chikkarayapuram, Chennai, 600069, India

    E. Nandhakumar

  4. Department of Chemistry, Manomaniam Sundaranar University, Tirunelveli, Tamil Nadu, 627012, India

    K. Muthu

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  1. R. Sundaram
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  2. V. Ganesh
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  4. K. Muthu
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Correspondence to R. Sundaram.

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The experimental protocol was approved by the Ministry of Social Justices and Empowerment, Government of India, and Institutional Animal Ethics Committee Guidelines (IAEC No: No. 12/09/2016).

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Sundaram, R., Ganesh, V., Nandhakumar, E. et al. Hepatoprotective and anti-inflammatory potential of chebulagic acid on carbon tetrachloride–induced hepatic fibrosis by antioxidative activities in rats. Comp Clin Pathol 30, 961–971 (2021). https://doi.org/10.1007/s00580-021-03295-0

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  • DOI: https://doi.org/10.1007/s00580-021-03295-0

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