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Effects of aflatoxins on some reproductive hormones and composition of buffalo’s milk

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Abstract

The aim of this study was to assess the effects of feeding aflatoxins on some reproductive hormones and on the composition of buffalo milk. Forty lactating buffaloes fed total mixed rations (TMR), part of which was contaminated by fungi secreting aflatoxin. The animals were divided into two equal groups: aflatoxin high group (AHG), animals were fed TMR containing aflatoxin > 21.2 ppb, and aflatoxin low one (ACG), buffaloes were fed on TMR containing aflatoxin < 5 ppb. Blood concentrations of follicle-stimulating hormone (FSH), luteinizing hormone (LH), progesterone, estradiol 17ß, cortisol, and prolactin were assayed. The physiochemical indices of milk samples including fat%, solid not fat (SNF%), Slats%, protein%, total solids%, and pH were measured. The aflatoxins detected in dietary ration and milk of the buffaloes were aflatoxin B1 (AFB1) and aflatoxin M1 (AFM1) respectively. The mean concentration of AFM1 in raw buffalo milk was (0.42 ± 0.043 ppb) in AHG, while there was (0.04 ± 0.002 ppb) in the ACG; the difference was significant (P < 0.05). Animals in AG, revealed a highly significant (P < 0.001) decrease in FSH, LH, and progesterone concentration compared with control animals. In contrast, estradiol 17 ß and cortisol increased significantly (P < 0.01) in AHG compared with ACG. Prolactin concentration showed a slight elevation with a non-significant difference. There were no significant differences in the milk parameters between the two groups. Aflatoxins disturb some endocrine functions of the reproductive organs, which could adversely affect buffalo’s productivity.

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Acknowledgments

The authors thank all workers in the Faculty of Veterinary Medicine, Aswan University for their help with this work.

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Authors and Affiliations

  1. Department of Theriogenology, Faculty of Veterinary Medicine, Aswan University, Aswan, Egypt

    Y. A. Amin

  2. Theriogenology, Faculty of Veterinary Medicine, Aswan University, Aswan, Egypt

    R. H. Mohamed

  3. Department of Food Hygiene and Control, Faculty of Veterinary Medicine, Aswan University, Aswan, Egypt

    A. M. Zakaria

  4. Clinic for Obstetrics, Gynecology and Andrology of Large and Small Animals, Vet Med, JLU Giessen, Giessen, Germany

    A. Wehrend

  5. Department of Theriogenology, Faculty Veterinary Medicine, Assiut University, Assiut, 71526, Egypt

    Hassan A. Hussein

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  1. Y. A. Amin
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  2. R. H. Mohamed
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  3. A. M. Zakaria
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  4. A. Wehrend
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  5. Hassan A. Hussein
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Correspondence to Hassan A. Hussein.

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All institutional and national guidelines for the care and use of animals were followed according to the Egyptian Medical Research Ethics Committee (no. 14 – 126).

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Highlights

Effects aflatoxicosis on some reproductive hormones and physicochemical composition of milk of buffaloes

Aflatoxins in dietary ration and milk of the buffaloes were AFB1 and AFM1 respectively.

Aflatoxins decrease FSH, LH, and progesterone concentration significantly

Aflatoxins increase estradiol and cortisol concentration significantly

Physiochemical indices of milk samples showed no recordable changes.

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Amin, Y.A., Mohamed, R.H., Zakaria, A.M. et al. Effects of aflatoxins on some reproductive hormones and composition of buffalo’s milk. Comp Clin Pathol 28, 1191–1196 (2019). https://doi.org/10.1007/s00580-019-03006-w

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  • DOI: https://doi.org/10.1007/s00580-019-03006-w

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