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Erythrocyte characteristics of the progenies of pure and reciprocal crosses of Pangasianodon hypophthalmus (Sauvage, 1878) and Clarias gariepinus (Burchell, 1822)

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Abstract

In this study, erythrocyte characteristic of pure and reciprocal crosses of African catfish Clarias gariepinus (Burchell, 1822) and Asian catfish Pangasianodon hypophthalmus (Sauvage, 1878) were evaluated. Blood smear was made from the progenies of C. gariepinus (CG), P. hypophthalmus (PH), Pangapinus (♀PH × ♂CG) and in two morphotypes (Clarias-like and Panga-like) of Clariothalmus (♀CG × ♂PH) obtained from similar breeding history. Erythrocyte measurement of the cell and nuclear axis was done under the microscope, while many other parameters were calculated. Result obtained suggests a preponderant maternal influence on the erythrocytes shape and size characteristics of the reciprocal crosses. Erythrocytes from progenies with C. gariepinus maternal origin was rounded in shape (not less than 90%) while those with P. hypophthalmus maternal origin had relatively high oval shaped erythrocytes (between 76 and 64%). Also, significant size variations were recorded for the different group. Principal component analysis and biplot of component loading of the trio comparison of hybrid progenies and the pure crosses suggest the possibility of discriminating the direction of hybridization of the reciprocal crosses. However, it was impossible to describe the hybridization status of the progenies assessed in this study. This approach may be a simple and viable alternative to molecular markers which characterizes maternal inheritance in hybrid crosses provided that there is significant shape difference between the fishes. Erythrocyte characterization coupled with morphological and cytogenetic studies is recommended for clearer understanding of the nature of hybrid progenies.

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Acknowledgements

The authors are indebted to the School of Fisheries and Aquaculture Science, Universiti Malaysia Terengganu, Malaysia for providing facilities and broodstock used in this study. We also acknowledge the immerse support and contributions of all technical staffs of the PPSPA hatchery department during the breeding trial for this study. This study is part of the first author’s Ph.D. research.

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Authors and Affiliations

  1. Department of Fisheries and Aquaculture, University of Agriculture, Makurdi, Nigeria

    V. T. Okomoda

  2. School of Fisheries and Aquaculture Sciences, Universiti Malaysia Terengganu, Kuala Terengganu, Terengganu, Malaysia

    V. T. Okomoda, I. C. C. Koh, A. Hassan, A. B. K. Khairul, R. Y. Rajamad & M. S. Shahreza

  3. Department of Technology and Industries, Prince of Songkla University, Pattani Campus, Hat Yai, Thailand

    T. Amornsakun

  4. Institute of Tropical Aquaculture (AQUATROP), Universiti Malaysia Terengganu, Kuala Terengganu, Terengganu, Malaysia

    A. D. Shuhaimi, M. R. Shafiq & M. S. Shahreza

Authors
  1. V. T. Okomoda
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  2. I. C. C. Koh
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  3. A. Hassan
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  4. T. Amornsakun
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  5. A. B. K. Khairul
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  6. R. Y. Rajamad
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  7. A. D. Shuhaimi
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  8. M. R. Shafiq
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  9. M. S. Shahreza
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Corresponding author

Correspondence to M. S. Shahreza.

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All applicable guidelines for the care and use of animals were followed for this study (which includes international, national, and/or institutional).

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Okomoda, V.T., Koh, I.C.C., Hassan, A. et al. Erythrocyte characteristics of the progenies of pure and reciprocal crosses of Pangasianodon hypophthalmus (Sauvage, 1878) and Clarias gariepinus (Burchell, 1822). Comp Clin Pathol 27, 301–312 (2018). https://doi.org/10.1007/s00580-017-2589-x

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