Abstract
The risk of miscarriage is enhanced by genetic and environmental factors. Several studies indicated that there was an association between endothelial nitric oxide synthase activity, implantation, and the maintenance of pregnancy. However, the results of these studies remain controversial. The aim of our study was to determine the association between recurrent spontaneous miscarriage (RSM) in Egyptian women and two common polymorphisms of the endothelial nitric oxide synthase (eNOS) gene: intron 4 and T-786C. The study was conducted on a total number of 100 participants, 50 patients with RSM and 50 age-matched healthy controls. Patients and controls were investigated for the two polymorphisms. Genotyping of the eNOS intron 4 polymorphisms was performed by PCR assay. Our results indicated that there was a significant difference in the frequencies of the genotypes between case and control groups for the polymorphisms in both intron 4 (odds ratio (OR) 5.09, 95 % confidence interval (CI) 2.091–12.396, P < 0.001) and T-786C (OR 7.389, 95 % CI 3.043–17.942, P < 0.001). In our study, the eNOS polymorphisms (in intron 4 and T-786C) were found to be significantly associated with increased risk of RSM in Egyptian women.
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References
Alfadhli S, AlTamimy B, AlSaeid K et al (2011) Endothelial nitric oxide synthase gene haplotype association with systemic lupus erythematosus. Lupus 20:700–708
Fan W, Li SW, Wang Y (2007) Association of genetic polymorphisms in endothelial nitric oxide synthase 3 gene with recurrent early spontaneous abortion. Zhonghua YiXue Yi Chuan Xue ZaZhi 24:23–26
Hingorani AD (2001) Polymorphisms in endothelial nitric oxide synthase and atherogenesis. Atherosclerosis 154:521–527
Karvela M, Papadopoulou S, Tsaliki E et al (2008) Endothelial nitric oxide synthase gene polymorphisms in recurrent spontaneous abortions. Arch Gynecol Obstet 278:349–352
Lapointe J, Roy M, St-Pierre L (2006) e al. Hormonal and spatial regulation of nitric oxide synthases (NOS) (neuronal NOS, inducible NOS, and endothelial NOS) in the oviducts. Endocrinology 147:560–561
Lee YH, Kim HJ, Rho YH et al (2004) Intron 4 polymorphism of the endothelial nitric oxide synthase gene is associated with the development of lupus nephritis. Lupus 13:188–191
Nakayama M, Yasue H, Yoshimura M et al (1999) T-786C mutation in the 5′-flanking region of the endothelial nitric oxide synthase gene is associated with coronary spasm. Circulation 99:2864–2870
Parveen F, Farid RM, Agrawal S (2011) Genetic analysis of eNOS gene polymorphisms in association with recurrent miscarriage among North Indian women. J SGPGIMS 11:1506–1510
Serrano NC, Paez C, Correa PA, Anaya JM (2004) Endothelial nitric oxide synthase gene polymorphism is associated with systemic lupus erythematosus. J Rheumatol 31:2163–2168
Shin SJ, Lee HH, Cha SH et al (2010) Endothelial nitric oxide synthase gene polymorphisms (786 T > C, 4a4b, 894G > T) and haplotypes in Korean patients with recurrent spontaneous abortion. Eur J Obstet Gynecol Reprod Biol 152:64–67
Su MT, Lin SH, Chen YC (2011) Genetic association studies of angiogenesis- and vasoconstriction-related genes in women with recurrent pregnancy loss: a systematic review and meta-analysis. Hum Reprod Update 152:64–67
Suryanarayana V, Rao L, Kanakavalli M et al (2006) Recurrent early pregnancy loss and endothelial nitric oxide synthase gene polymorphisms. Arch Gynecol Obstet 274:119–124
Tempfer C, Unfried G, Zeillinger R et al (2001) Endothelial nitric oxide synthase gene polymorphism in women with idiopathic recurrent miscarriage. Hum Reprod 16:1644–1647
Tseng LH, Chen PJ, Lin MT et al (2002) Simultaneous genotyping of single nucleotide polymorphisms in the IL-6, IL-10, TNF alpha and TNF beta genes. Tissue Antigens 59:280–286
Zammiti W, Mtiraoui N, Mahjoub T (2008) Lack of consistent association between endothelial nitric oxide synthase gene polymorphisms, homocysteine levels and recurrent pregnancy loss in Tunisian women. Am J Reprod Immunol 59:139–145
Zygmunt M, Herr F, Keller-Sch€onwein S et al (2002) Human chorionic gonadotropin (HCG) is an angiogenetic factor for uterine endothelial cells in vitro. J Clin Endocrinol Metab 87:5290–5296
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Abulata, N.N., AbdalWahab, D.F. & Osman, O.M. Endothelial nitric oxide synthase gene polymorphism in a cohort of Egyptian women with recurrent spontaneous miscarriage. Comp Clin Pathol 24, 629–634 (2015). https://doi.org/10.1007/s00580-014-1957-z
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DOI: https://doi.org/10.1007/s00580-014-1957-z