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Dynamics of post-translationally modified histones during barley pollen embryogenesis in the presence or absence of the epi-drug trichostatin A

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Key message

Improving pollen embryogenesis.

Abstract

Despite the agro-economic importance of pollen embryogenesis, the mechanisms underlying this process are still poorly understood. We describe the dynamics of chromatin modifications (histones H3K4me2, H3K9ac, H3K9me2, and H3K27me3) and chromatin marks (RNA polymerase II CDC phospho-Ser5, and CENH3) during barley pollen embryogenesis. Immunolabeling results show that, in reaction to stress, immature pollen rapidly starts reorganizing several important chromatin modifications indicative of a change in cell fate. This new chromatin modification pattern was accomplished within 24 h from whereon it remained unaltered during subsequent mitotic activity. This indicates that cell fate transition, the central element of pollen embryogenesis, is completed early on during the induction process. Application of the histone deacetylase inhibitor trichostatin A stimulated pollen embryogenesis when used on pollen with a gametophytic style chromatin pattern. However, when this drug was administered to embryogenic pollen, the chromatin markers reversed toward a gametophytic profile, embryogenesis was halted and all pollen invariably died.

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Acknowledgements

We would like to thank Katrin Kumke, Ingrid Otto and Monika Wiesner for excellent technical support and Dr. Marek Marzec for helpful discussions. The first author was supported by the Siemens/DAAD (Deutscher Akademischer Austauschdienst) postgraduate program with Grant A/11/71982.

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Authors and Affiliations

  1. Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Gatersleben, Germany

    Pooja Pandey, Diaa S. Daghma, Andreas Houben, Jochen Kumlehn, Michael Melzer & Twan Rutten

  2. Imperial College London, London, UK

    Pooja Pandey

  3. Institute for Experimental Trauma Surgery, Justus-Liebig University of Giessen, Giessen, Germany

    Diaa S. Daghma

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  1. Pooja Pandey
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  2. Diaa S. Daghma
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  3. Andreas Houben
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  4. Jochen Kumlehn
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  5. Michael Melzer
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  6. Twan Rutten
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Correspondence to Twan Rutten.

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The authors declare that they have no conflict of interest.

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Communicated by Dolf Weijers.

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Pandey, P., Daghma, D.S., Houben, A. et al. Dynamics of post-translationally modified histones during barley pollen embryogenesis in the presence or absence of the epi-drug trichostatin A. Plant Reprod 30, 95–105 (2017). https://doi.org/10.1007/s00497-017-0302-5

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  • DOI: https://doi.org/10.1007/s00497-017-0302-5

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