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Production of conidia of the entomopathogenic fungus Metarhizium anisopliae ICB 425 in a tray bioreactor

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Abstract

The use of Metarhizium anisopliae as a bioinsecticide is steeply increasing worldwide. However, to reduce the production costs, it is necessary to develop sophisticated techniques for conidia production. This work aimed to use a tray bioreactor to produce conidia of M. anisopliae ICB-425 in long rice and find the limiting bed depth in which the production is still viable. Experiments have been carried out to assess the influence of the air temperature and relative humidity on the spore concentration in order to determine the limiting temperature. Two scales of bioreactors in plastic packages have been used, containing 10 and 500 g of rice, and the results were similar. In the tray bioreactor, the bed depths of 2, 4 and 6 cm have been used, corresponding to the dry rice weights of 1, 2 and 3 kg, respectively, and the results were similar to the ones in plastic packages. A one-phase heat transfer model has been used to foresee the maximum temperature within the bed and the results agreed fairly well with the experimental ones. Using the model, a bed depth of 7 cm was found to be the limit for the tray bioreactor. The results obtained are very promising for the mass production of conidia of M. anisopliae at lower costs and with more effective control.

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Acknowledgements

The authors would like to thank to the São Paulo Research Foundation (FAPESP, Proc. 2006/55641-7, 2018/00996-2) for the financial support and CAPES for the scholarship.

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Authors and Affiliations

  1. Food Engineering and Technology Department, Institute of Biosciences, Letters and Exact Sciences, São Paulo State University (UNESP), Cristóvão Colombo 2265, Jardim Nazareth, São José Do Rio Preto, SP, 15054-000, Brazil

    Lucas Portilho da Cunha, Isabella de Cenço Lopes & João Cláudio Thoméo

  2. Chemical Engineering Department, Federal University of São Carlos (UFSCar), Rod. Washington Luiz, km 235, SP 310, Bairro Monjolinho, São Carlos, SP, 13565-905, Brazil

    Fernanda Perpétua Casciatori

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  1. Lucas Portilho da Cunha
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Correspondence to Lucas Portilho da Cunha.

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Lucas Portilho da Cunha, Fernanda Perpétua Casciatori, Isabella de Cenço Lopes and João Cláudio Thoméo declare that they have no conflict of interest.

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da Cunha, L.P., Casciatori, F.P., de Cenço Lopes, I. et al. Production of conidia of the entomopathogenic fungus Metarhizium anisopliae ICB 425 in a tray bioreactor. Bioprocess Biosyst Eng 42, 1757–1768 (2019). https://doi.org/10.1007/s00449-019-02172-z

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