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Efficient and low-cost alternative of lipase concentration aiming at the application in the treatment of waste cooking oils

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Abstract

In this study, we evaluated the concentration of lipases from Aspergillus niger using efficient and low-cost methods aiming at application in the treatment of waste cooking oils. The change in ionic strength of the medium by the addition of salt and precipitation with ethanol increased the specific activity from 2.90 to 28.50 U/mg, resulting in a purification factor of 9.82-fold. The use of acetone resulted in a specific activity of 33.63 U/mg, resulting in a purification factor of 11.60-fold. After that, the concentrated lipase was used in the hydrolysis of waste cooking oil and 753.07 and 421.60 µmol/mL of free fatty acids were obtained for the enzyme precipitated with ethanol and acetone, respectively. The hydrolysis of waste cooking oil catalyzed by homemade purified lipase in ultrasonic media can be considered a pretreatment of oil by converting a significant amount of triglycerides into free fatty acids.

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Acknowledgements

CAPES, FAPERGS, and CNPq are acknowledged for financial support.

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Authors and Affiliations

  1. Universidade Federal da Fronteira Sul-UFFS, Rodovia ERS 135, km 72, no 200, Erechim, RS, Brazil

    Karina P. Preczeski, Angela B. Kamanski, Thamarys Scapini, Aline F. Camargo, Tatiani A. Modkoski, Vanusa Rossetto, Bruno Venturin, Simone M. Golunski, Altemir J. Mossi & Helen Treichel

  2. Departamento de Engenharia Química e de Alimentos, Universidade Federal de Santa Catarina, Campus Universitário, Florianópolis, SC, 88800-000, Brazil

    Jéssica Mulinari

Authors
  1. Karina P. Preczeski
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  2. Angela B. Kamanski
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  3. Thamarys Scapini
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  4. Aline F. Camargo
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  5. Tatiani A. Modkoski
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  6. Vanusa Rossetto
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  7. Bruno Venturin
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  8. Jéssica Mulinari
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  9. Simone M. Golunski
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  10. Altemir J. Mossi
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  11. Helen Treichel
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Correspondence to Helen Treichel.

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Preczeski, K.P., Kamanski, A.B., Scapini, T. et al. Efficient and low-cost alternative of lipase concentration aiming at the application in the treatment of waste cooking oils. Bioprocess Biosyst Eng 41, 851–857 (2018). https://doi.org/10.1007/s00449-018-1919-y

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  • DOI: https://doi.org/10.1007/s00449-018-1919-y

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