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Comparative study of multi-enzyme production from typical agro-industrial residues and ultrasound-assisted extraction of crude enzyme in fermentation with Aspergillus japonicus PJ01

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Abstract

Submerged fermentation (SmF) and solid-state fermentation (SSF) of Aspergillus japonicus PJ01 for multi-enzyme complexes (MEC) production were comparatively studied. The results showed that orange peel and wheat bran were the best substrates for MEC production in SmF and SSF, respectively. After 72 h of cultivation under SmF, the maximal pectinase, CMCase, and xylanase activities reached 2610, 85, and 335 U/gds (units/gram dry substrate), respectively; while after 72 h of cultivation under SSF, these three enzymes’ activities reached 966, 58, and 1004 U/gds, respectively. Effects of ultrasound on extraction of crude enzymes from SSF medium were determined, the maximal activities of pectinase, CMCase, and xylanase increased to 1.20, 1.48, and 1.30-fold, respectively. Apparent different mycelia growths of SSF and SmF were observed by scanning electron microscopy; and different isoforms of the crude enzyme extracts from SSF and SmF were presented by zymogram analysis.

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Acknowledgments

This work was supported by a Grant from the National High Technology Research and Development Program of China (863 Program) (No. 2011AA100804), Hunan Provincial Innovation Foundation for Postgraduate (CX2013B084), and Open-End Fund for the Valuable and Precision Instruments of Central South University (CSUZC2014003).

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Authors and Affiliations

  1. School of Minerals Processing and Bioengineering, Central South University, Changsha, 410083, China

    Pei-jun Li, Jin-lan Xia & Zhen-yuan Nie

  2. Hunan Academy of Agricultural Sciences, Changsha, 410125, China

    Yang Shan

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  1. Pei-jun Li
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Correspondence to Jin-lan Xia.

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Li, Pj., Xia, Jl., Shan, Y. et al. Comparative study of multi-enzyme production from typical agro-industrial residues and ultrasound-assisted extraction of crude enzyme in fermentation with Aspergillus japonicus PJ01. Bioprocess Biosyst Eng 38, 2013–2022 (2015). https://doi.org/10.1007/s00449-015-1442-3

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