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Whole slurry saccharification and fermentation of maleic acid-pretreated rice straw for ethanol production

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Abstract

We evaluated the feasibility of whole slurry (pretreated lignocellulose) saccharification and fermentation for producing ethanol from maleic acid-pretreated rice straw. The optimized conditions for pretreatment were to treat rice straw at a high temperature (190 °C) with 1 % (w/v) maleic acid for a short duration (3 min ramping to 190 °C and 3 min holding at 190 °C). Enzymatic digestibility (based on theoretical glucose yield) of cellulose in the pretreated rice straw was 91.5 %. Whole slurry saccharification and fermentation of pretreated rice straw resulted in 83.2 % final yield of ethanol based on the initial quantity of glucan in untreated rice straw. These findings indicate that maleic acid pretreatment results in a high yield of ethanol from fermentation of whole slurry even without conditioning or detoxification of the slurry. Additionally, the separation of solids and liquid is not required; therefore, the economics of cellulosic ethanol fuel production are significantly improved. We also demonstrated whole slurry saccharification and fermentation of pretreated lignocellulose, which has rarely been reported.

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Acknowledgments

This work was supported by grants from the Ministry of Trade, Industry & Energy (10047873 and 10049674). A Korea University grant through the Korea University Food Safety Hall for the Institute of Biomedical Science and Food Safety is acknowledged.

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Authors and Affiliations

  1. Department of Biotechnology, Korea University Graduate School, Seoul, 136-713, Republic of Korea

    Young Hoon Jung, Hyun Min Park & Kyoung Heon Kim

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  1. Young Hoon Jung
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  2. Hyun Min Park
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  3. Kyoung Heon Kim
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Correspondence to Kyoung Heon Kim.

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Jung, Y.H., Park, H.M. & Kim, K.H. Whole slurry saccharification and fermentation of maleic acid-pretreated rice straw for ethanol production. Bioprocess Biosyst Eng 38, 1639–1644 (2015). https://doi.org/10.1007/s00449-015-1405-8

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  • DOI: https://doi.org/10.1007/s00449-015-1405-8

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