Abstract
A sensitive electrochemical immunosensor based on NiAl-layered double hydroxide/graphene nanocomposites (NiAl-LDH/G) and hollow gold nanospheres (HGNs) was proposed for chlorpyrifos detection. The NiAl-LDH/G was prepared using a conventional coprecipitation process and reduction of the supporting graphene oxide. Subsequently, the nanocomposites were dispersed with chitosan (CS). The NiAl-LDH/G possessed good electrochemical behavior and high binding affinity to the electrode. The high surface areas of HGNs and the vast aminos and hydroxyls of CS provided a platform for the covalently crosslinking of antibody. Under optimal conditions, the immunosensor exhibited a wide linear range from 5 to 150 μg/mL and from 150 to 2 μg/mL, with a detection limit of 0.052 ng/mL. The detection results showed good agreement with standard gas chromatography method. The constructed immunosensor exhibited good reproducibility, high specificity, acceptable stability and regeneration performance, which provided a new promising tool for chlorpyrifos detection in real samples.
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Acknowledgments
This work was supported by the National Natural Science Foundation of China (No. 30972055, 31101286, 31471641), Agricultural Science and Technology Achievements Transformation Fund Projects of the Ministry of Science and Technology of China (No. 2011GB2C60020), Special project of independent innovation of Shandong Province (2014CGZH0703) and Shandong Provincial Natural Science Foundation, China (No. Q2008D03, ZR2014CM009).
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L. Qiao and Y. Guo contributed equally to this work.
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Qiao, L., Guo, Y., Sun, X. et al. Electrochemical immunosensor with NiAl-layered double hydroxide/graphene nanocomposites and hollow gold nanospheres double-assisted signal amplification. Bioprocess Biosyst Eng 38, 1455–1468 (2015). https://doi.org/10.1007/s00449-015-1388-5
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DOI: https://doi.org/10.1007/s00449-015-1388-5