Abstract
In this study, a straight diagonal-pitched blade stirrer was designed, built and characterized in a 5-L fermenter. Compared with the six straight blade Rushton turbine, the power consumption of the new stirrer is lower at a given speed under conditions of no ventilation. The oxygen transference is poorer at the same agitation speed in the cultivation conditions and scales investigated, which confirms that the shear stress of the new stirrer is lower and the gas dispersion is weaker. The new stirrer was installed in a 5-L bioreactor and evaluated in submerged fermentation of the edible fungus Pleurotus ostreatus. The results showed that the maximum dry weight of mycelium is increased by 47 % and reached 7.47 g/L, and the maximum laccase activity is increased by 15 % up to 2,277 U/L. Glucose consumption was also found to be relatively faster. The power consumption is 2.8 % lower than that of the Rushton turbine.
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Abbreviations
- Rushton turbine:
-
Six straight blade Rushton turbine
- DO:
-
Dissolved oxygen
- P o :
-
The mixing power
- Ρ :
-
Medium density
- N :
-
The stirring speed
- N p :
-
The power number
- D :
-
The agitator diameter
- K L a :
-
Volumetric mass transfer coefficient
- C L * :
-
Saturated oxygen concentration
- C L :
-
Dissolved oxygen concentration at time (t)
- C 0 :
-
Initial dissolved oxygen concentration
- μ :
-
The apparent viscosity
- γ :
-
The kinematic viscosity
- u t :
-
The impeller tip speed
- γ max :
-
The maximal shear stress
- γ ave :
-
The average shear stress
- Re:
-
Reynolds number
- V :
-
Voltage
- I :
-
Current
- ABTS:
-
2,2-azinobis-3-ethylbenzthiazoline-6-sulfonate
- PDA:
-
Potato dextrose agar
- MDW:
-
Mycelium dry weight
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Acknowledgments
We thank Robert K. Thomas FRS from University of Oxford for his contribution in improving the manuscript. This work was financially supported by the Natural Science Foundation of China (No. 41276135, 31172010 and 30800775), Program for New Century Excellent Talents in University (NCET-13-1031) and the Fundamental Research Funds for the Central Universities (13CX02063A).
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Zhu, H., Sun, J., Tian, B. et al. A novel stirrer design and its application in submerged fermentation of the edible fungus Pleurotus ostreatus . Bioprocess Biosyst Eng 38, 509–516 (2015). https://doi.org/10.1007/s00449-014-1290-6
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DOI: https://doi.org/10.1007/s00449-014-1290-6