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Light acclimation optimizes leaf functional traits despite height-related constraints in a canopy shading experiment

  • Physiological ecology - Original research
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Abstract

Within-canopy gradients of leaf functional traits have been linked to both light availability and vertical gradients in leaf water potential. While observational studies can reveal patterns in leaf traits, within-canopy experimental manipulations can provide mechanistic insight to tease apart multiple interacting drivers. Our objectives were to disentangle effects of height and light environment on leaf functional traits by experimentally shading branches along vertical gradients within a sugar maple (Acer saccharum) forest. Shading reduced leaf mass per area (LMA), leaf density, area-based leaf nitrogen (Narea), and carbon:nitrogen (C:N) ratio, and increased mass-based leaf nitrogen (Nmass), highlighting the importance of light availability on leaf morphology and chemistry. Early in the growing season, midday leaf water potential (Ψmid), LMA, and Narea were driven primarily by height; later in the growing season, light became the most important driver for LMA and Narea. Carbon isotope composition (δ13C) displayed strong, linear correlations with height throughout the growing season, but did not change with shading, implying that height is more influential than light on water use efficiency and stomatal behavior. LMA, leaf density, Nmass, C:N ratio, and δ13C all changed seasonally, suggesting that leaf ageing effects on leaf functional traits are equally as important as microclimatic conditions. Overall, our results indicate that: (1) stomatal sensitivity to vapor pressure deficit or Ψmid constrains the supply of CO2 to leaves at higher heights, independent of light environment, and (2) LMA and Narea distributions become functionally optimized through morphological acclimation to light with increasing leaf age despite height-related constraints.

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Acknowledgments

We thank Ashley Coble, Alex Collins, Mickey Jarvi, Dr. Kevyn Juneau, Alida Mau, and Brittany Vanderwall for their assistance in the lab and field. We also thank two anonymous reviewers and Dr. Christian Messier for helpful comments on the manuscript. We also thank ABEE, Inc. for installing a safe and effective zip-line system. Research was sponsored by the National Institute of Food and Agriculture US Department of Agriculture McIntire-Stennis Cooperative Forestry Research Program (grant # 32100-06098) and the Ecosystem Science Center at Michigan Technological University.

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    Adam P. Coble & Molly A. Cavaleri

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Correspondence to Adam P. Coble.

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Coble, A.P., Cavaleri, M.A. Light acclimation optimizes leaf functional traits despite height-related constraints in a canopy shading experiment. Oecologia 177, 1131–1143 (2015). https://doi.org/10.1007/s00442-015-3219-4

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