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Toll-like receptor 10 has a role in human macrophage response against Streptococcus pneumoniae

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Abstract

Toll-like receptors (TLRs) are evolutionarily conserved pathogen-associated molecular pattern recognition receptors, and play a critical role in early response against invading pathogens. Even though TLRs have been widely studied, very little is known about the expression and function of TLR10. Till date, neither any data are available on expression of TLR10 in human lungs nor there is any information on function of TLR10 in macrophages. Streptococcus pneumoniae are Gram-positive, alpha-hemolytic, and major causative agent of pneumonia, ear infections, sinus infections, and meningitis. We examined the role of TLR10 in innate immune response to S. pneumoniae infection in U937 cell line-derived human macrophages. We found a significant increase in TLR10 mRNA and protein expression in S. pneumoniae challenged macrophages. TLR10 knockdown resulted in significant reduction of IL-1β, IL-8, IL-17, and TNF-α but not IL-10 expression in infected macrophages. TLR10 knockdown in macrophages reduced nuclear translocation of NF-κB during S. pneumoniae challenge but did not affect the phagocytosis of the bacteria. Taken together, we report the first data on TLR10’s role in macrophage response against S. pneumoniae.

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Acknowledgements

Authors appreciate the technical support of Daryoush Hajinezhad and Shanna Banman. The work was supported through a Discovery Grant from Natural Sciences and Engineering Research Council of Canada to Dr. Baljit Singh. Dr. Yadu Balachandran was supported through a scholarship from Natural Sciences and Engineering Research Council’s Integrated Training Program in Infectious Diseases, Food Safety and Public Policy.

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Authors and Affiliations

  1. Pulmonary Pathobiology Lab, Department of Veterinary Biomedical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, SK, Canada

    Yadu Balachandran & Baljit Singh

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  1. Yadu Balachandran
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  2. Baljit Singh
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Contributions

Y.B. and B.S. conceived the idea, R.K. provided the human samples and directions for immunohistological analysis, and Y.B. performed research. Y.B. and B.S. wrote the manuscript.

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Correspondence to Baljit Singh.

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This manuscript formed part of PhD thesis of Dr. Yadu Balachandaran submitted to the University of Saskatchewan.

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The authors declare no competing interests.

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Balachandran, Y., Singh, B. Toll-like receptor 10 has a role in human macrophage response against Streptococcus pneumoniae. Cell Tissue Res 390, 51–57 (2022). https://doi.org/10.1007/s00441-022-03671-4

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  • DOI: https://doi.org/10.1007/s00441-022-03671-4

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