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Ablation of the sperm-associated antigen 11A (SPAG11A) protein by active immunization promotes epididymal oncogenesis in the rat

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Abstract

Incidence of cancer in the epididymis is very rare. It is proposed that proteins specific to this organ may contribute to this unique property. We previously demonstrated that siRNA–mediated knockdown of SPAG11A mRNA resulted in increased proliferation of epididymal epithelial cells, whereas overexpression of this gene caused reduced proliferation in immortalized cell lines. In this study, we evaluated the oncogenesis-related anatomical and transcriptome changes in the epididymis of SPAG11A–immunized rats challenged with a low dose of diethyl nitrosamine (DEN). DEN treatment or SPAG11A immunization alone did not cause any histopathological changes in the epididymis. Interestingly, indications of oncogenesis were observed in SPAG11A–immunized + DEN–treated rats. Using high throughput sequencing, we observed that 3549 transcripts that were differentially expressed in the caput epididymis of DEN only–treated rats displayed similar differential expression in the caput epididymis of SPAG11A–immunized rats, indicating that the microenvironment that contributes to oncogenesis sets in when SPAG11A protein is ablated. Differential expression of genes that are involved in 10 major cancer related pathways was also analyzed. Majority of the genes related to these pathways that were differentially expressed in the caput epididymis of DEN only–treated rats also showed similar pattern in the caput epididymis of SPAG11A–immunized rats. For the first time, results of our study demonstrate that ablation of SPAG11A by active immunization renders the epididymis susceptible to oncogenesis and that this protein may be one of the factors that contributes to the rarity of epididymal cancer.

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Acknowledgements

Funding from Science and Engineering Research Board (SERB), Government of India, in the form of research project (EMR/2016/000791) is highly acknowledged. We thank the facilities extended by UGC-SAP, UGC-CAS, DBT-CREBB, DST-PURSE, UGC-UPE-II, and FIST programs at School of Life Sciences, University of Hyderabad. SK received the junior research fellowship from Government of India.

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  1. Department of Animal Biology, University of Hyderabad, Hyderabad – 500046, India

    Kumari Sangeeta & Suresh Yenugu

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  1. Kumari Sangeeta
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  2. Suresh Yenugu
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SK carried out the experimental work. SY conceptualized the ideas, wrote the manuscript, and provided the reagents and other facilities.

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Correspondence to Suresh Yenugu.

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All the animal experiments in this study were conducted with the approval of Institutional Animal Ethics Committee (IAEC) of University of Hyderabad (permit no. UH/IAEC/SY/2016-II/P13, dated 7th March, 2017; UH/IAEC/SY/2021–1/20, dated 27th January, 2021).

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Sangeeta, K., Yenugu, S. Ablation of the sperm-associated antigen 11A (SPAG11A) protein by active immunization promotes epididymal oncogenesis in the rat. Cell Tissue Res 389, 115–128 (2022). https://doi.org/10.1007/s00441-022-03623-y

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