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The mRNA decapping protein 2 (DCP2) is a major regulator of developmental events in Drosophila—insights from expression paradigms

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Abstract

The Drosophila genome codes for two decapping proteins, DCP1 and DCP2, out of which DCP2 is the active decapping enzyme. The present endeavour explores the endogenous promoter firing, transcript and protein expression of DCP2 in Drosophila wherein, besides a ubiquitous expression across development, we identify an active expression paradigm during dorsal closure and a plausible moonlighting expression in the Corazonin neurons of the larval brain. We also demonstrate that the ablation of DCP2 leads to embryonic lethality and defects in vital morphogenetic processes whereas a knockdown of DCP2 in the Corazonin neurons reduces the sensitivity to ethanol in adults, thereby ascribing novel regulatory roles to DCP2. Our findings unravel novel putative roles for DCP2 and identify it as a candidate for studies on the regulated interplay of essential molecules during early development in Drosophila, nay the living world.

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Acknowledgements

The authors acknowledge the fly community for generously providing the fly stocks. We thank Prof. B. J. Rao, TIFR, Mumbai for providing the TRE-JNK/CyO stock; Prof. Gaiti Hasan, NCBS, Bangalore, for providing the sNPF-GAL4, Dilp2-GAL4 and Crz-GAL4/CyO stocks and Prof. Utpal Banerjee, UCLA, for providing the G-TRACE/CyO flies. We duly acknowledge the National Facility for Laser Scanning Confocal Microscopy, Department of Zoology, Banaras Hindu University. The equipment facility supported by UGC-CAS, DST-FIST, and IoE to Department of Zoology are duly acknowledged. We sincerely acknowledgeNabarun Nandy for the assistance and valuable discussions. We sincerely thank Department of Science and Technology (DST) for providing fellowship to RK and support to JKR.

Funding

 Financial support received from Department of Science and Technology, New Delhi in the form of fellowship to RK is acknowledged.

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  1. Cytogenetics Laboratory, Department of Zoology, Institute of Science, Banaras Hindu University, Uttar Pradesh, Varanasi, 221005, India

    Rohit Kunar & Jagat Kumar Roy

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  1. Rohit Kunar
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RK: conceptualization, resources, methodology, investigation, data curation, formal analysis and interpretation, writing the manuscript. JKR: supervision, resources, writing the manuscript.

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Correspondence to Jagat Kumar Roy.

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Kunar, R., Roy, J.K. The mRNA decapping protein 2 (DCP2) is a major regulator of developmental events in Drosophila—insights from expression paradigms. Cell Tissue Res 386, 261–280 (2021). https://doi.org/10.1007/s00441-021-03503-x

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