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Ouabain-induced activation of phospholipase C zeta and its contributions to bovine sperm capacitation

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Abstract

The sperm-derived oocyte activating factor, phospholipase C zeta (PLC ζ), is the only PLC isoform reported in cattle. The objectives were to (1) localize PLC ζ in fresh and capacitated bovine sperm and (2) investigate the activation of PLC ζ during bull sperm capacitation and contributions of PLC activity to this process. We confirmed interaction of testis-specific isoform of Na/K-ATPase (ATP1A4) with PLC ζ (immunolocalization and immunoprecipitation) and tyrosine phosphorylation (immunoprecipitation) of PLC ζ (a post-translational protein modification commonly involved in activation of PLC in somatic cells) during capacitation. Furthermore, incubation of sperm under capacitating conditions upregulated PLC-mediated hyperactivated motility, tyrosine phosphoprotein content, acrosome reaction, and F-actin formation (flow cytometry), implying that PLC activity is enhanced during capacitation and contributing to these capacitation processes. In conclusion, we inferred that PLC ζ is activated during capacitation by tyrosine phosphorylation through a mechanism involving ATP1A4, contributing to capacitation-associated biochemical events.

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Acknowledgements

This study received funding from the Natural Sciences and Engineering Research Council (NSERC) of Canada (Grant # RGPN-2020-04585 to JT). We thank Alta Genetics Inc., Calgary, AB, for providing semen samples for the study.

Funding

This study received funding from the Natural Sciences and Engineering Research Council (NSERC) of Canada (Grant No. RGPIN-2020-04585).

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Authors and Affiliations

  1. Department of Production Animal Health, Faculty of Veterinary Medicine, University of Calgary, Calgary, CAL, T2N 4N1, Canada

    Veena Unnikrishnan, John P Kastelic & Jacob C Thundathil

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  1. Veena Unnikrishnan
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  2. John P Kastelic
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  3. Jacob C Thundathil
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Correspondence to Jacob C Thundathil.

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The study reported herein was approved by the University of Calgary Institutional Animal Care and Use Committee (protocol number: AC170119).

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The authors declare no competing interests.

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Unnikrishnan, V., Kastelic, J.P. & Thundathil, J.C. Ouabain-induced activation of phospholipase C zeta and its contributions to bovine sperm capacitation. Cell Tissue Res 385, 785–801 (2021). https://doi.org/10.1007/s00441-021-03455-2

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  • DOI: https://doi.org/10.1007/s00441-021-03455-2

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