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Regeneration of axotomized olfactory neurons in young and adult locusts quantified by fasciclin I immunofluorescence

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Abstract

The olfactory pathway of the locust Locusta migratoria is characterized by a multiglomerular innervation of the antennal lobe (AL) by olfactory receptor neurons (ORNs). After crushing the antenna and thereby severing ORN axons, changes in the AL were monitored. First, volume changes were measured at different times post-crush with scanning laser optical tomography in 5th instar nymphs. AL volume decreased significantly to a minimum volume at 4 days post-crush, followed by an increase. Second, anterograde labeling was used to visualize details in the AL and antennal nerve (AN) during de- and regeneration. Within 24 h post-crush (hpc) the ORN fragments distal to the lesion degenerated. After 48 hpc, regenerating fibers grew through the crush site. In the AL, labeled ORN projections disappeared completely and reappeared after a few days. A weak topographic match between ORN origin on the antenna and the position of innervated glomeruli that was present in untreated controls did not reappear after regeneration. Third, the cell surface marker fasciclin I that is expressed in ORNs was used for quantifying purposes. Immunofluorescence was measured in the AL during de- and regeneration in adults and 5th instar nymphs: after a rapid but transient, decrease, it reappeared. Both processes happen faster in 5th instar nymphs than in adults.

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Acknowledgements

The study was partially funded by the German Federal Ministry for Education and Research (BMBF grant 031L0062A).

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Authors and Affiliations

  1. Division of Cell Biology, University of Veterinary Medicine Hannover, Bischofsholer Damm 15/102, D-30173, Hannover, Germany

    Hannah Wasser, Alexandra Biller, Gerd Bicker & Michael Stern

  2. Biomedical Optics Department, Laser Zentrum Hannover e. V., Hollerithallee 8, D-30419, Hannover, Germany

    Georgios Antonopoulos & Heiko Meyer

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  1. Hannah Wasser
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  2. Alexandra Biller
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Correspondence to Michael Stern.

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Wasser, H., Biller, A., Antonopoulos, G. et al. Regeneration of axotomized olfactory neurons in young and adult locusts quantified by fasciclin I immunofluorescence. Cell Tissue Res 368, 1–12 (2017). https://doi.org/10.1007/s00441-016-2560-1

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  • DOI: https://doi.org/10.1007/s00441-016-2560-1

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