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Regulation of PPARγ/CPT-1 expression ameliorates cochlear hair cell injury by regulating cellular lipid metabolism and oxidative stress

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A Correction to this article was published on 25 April 2023

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Abstract

This study aimed to investigate the protective effects of PPARγ/CPT-1 regulation on cisplatin-induced cochlear hair cell injury. The viability, apoptosis and mitochondrial membrane potential of cisplatin-induced HEI-OC1 cells were determined by CCK-8 assay, TUNEL and JC-1 staining, respectively. The oxidative stress and lipid metabolism were detected by the assay kits of MDA, ROS, SOD, CAT, TG and FFA. The transfection efficiency of overexpression (OV)-PPARG and OV-CPT1A was examined by RT-qPCR and the expressions of apoptosis- and lipid metabolism-related proteins were detected by western blot. As a result, cisplatin with varying concentrations (5, 10, 30 μM) suppressed the viability, promoted the apoptosis and hindered the mitochondrial function of HEI-OC1 cells, accompanied with up-regulated expressions of Bax and cleaved caspase-3 and down-regulated expression of Bcl-2. The oxidative stress was aggravated and lipid metabolism was inhibited by cisplatin (5, 10, 30 μM) induction, evidenced by the increased levels of MDA, ROS, TG, FFA and the decreased levels of SOD and CAT. Overexpression of PPARG or CPT1A could improve the viability, mitochondrial function, lipid metabolism and suppress the oxidative stress and apoptosis of cisplatin-induced HEI-OC1 cells. In conclusion, up-regulation of PPARG or CPT1A ameliorated cochlear hair cell injury by improving cellular lipid metabolism and inhibiting oxidative stress.

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The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.

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  1. Xiaorong Niu and Peng Han these authors contributed equally.

Authors and Affiliations

  1. Department of Otorhinolaryngology Head and Neck Surgery, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, 710061, Shaanxi, China

    Xiaorong Niu, Peng Han, Junsong Liu, Ting Zhang, Baiya Li, Xiaoyan Ma & Qun Wu

  2. Department of Otorhinolaryngology Head and Neck Surgery, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an, 710004, Shaanxi, China

    Zichen Chen

  3. Department of Neurosurgery, The First Affiliated Hospital of Xi’an Jiaotong University, #227 Yanta West Road, Xi’an, 710061, Shaanxi, China

    Xudong Ma

Authors
  1. Xiaorong Niu
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  2. Peng Han
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  4. Zichen Chen
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Contributions

XDM: designed and conceived the study. XN and PH: conducted the experiments. JL, ZC, TZ, BL, XIM and QW: helped them to collect and analyze the data. XN and PH: drafted the manuscript which was polished by XDM. All authors have read and approved the final manuscript.

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Correspondence to Xudong Ma.

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Communicated by Martine Collart.

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Niu, X., Han, P., Liu, J. et al. Regulation of PPARγ/CPT-1 expression ameliorates cochlear hair cell injury by regulating cellular lipid metabolism and oxidative stress. Mol Genet Genomics 298, 473–483 (2023). https://doi.org/10.1007/s00438-023-01993-8

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