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The phenotypic and molecular assessment of the non-conserved Arabidopsis MICRORNA163/S-ADENOSYL-METHYLTRANSFERASE regulatory module during biotic stress

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Abstract

In plants, microRNAs (miRNAs) have evolved in parallel to the protein-coding genes that they target for expression regulation, and miRNA-directed gene expression regulation is central to almost every cellular process. MicroRNA, miR163, is unique to the Arabidopsis genus and is processed into a 24-nucleotide (nt) mature small regulatory RNA (sRNA) from a single precursor transcript transcribed from a single locus, the MIR163 gene. The MIR163 locus is a result of a recent inverted duplication event of one of the five closely related S-ADENOSYL-METHYLTRANSFERASE genes that the mature miR163 sRNA targets for expression regulation. Currently, however, little is known about the role of the miR163/S-ADENOSYL-METHYLTRANSFERASE regulatory module in response to biotic stress. Here, we document the expression domains of MIR163 and the S-ADENOSYL-METHYLTRANSFERASE target genes following fusion of their putative promoter sequences to the β-glucuronidase (GUS) reporter gene and subsequent in planta expression. Further, we report on our phenotypic and molecular assessment of Arabidopsis thaliana plants with altered miR163 accumulation, namely the mir163-1 and mir163-2 insertion knockout mutants and the miR163 overexpression line, the MIR163-OE plant. Finally, we reveal miR163 accumulation and S-ADENOSYL-METHYLTRANSFERASE target gene expression post treatment with the defence elicitors, salicylic acid and jasmonic acid, and following Fusarium oxysporum infection, wounding, and herbivory attack. Together, the work presented here provides a comprehensive new biological insight into the role played by the Arabidopsis genus-specific miR163/S-ADENOSYL-METHYLTRANSFERASE regulatory module in normal A. thaliana development and during the exposure of A. thaliana plants to biotic stress.

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Authors and Affiliations

  1. School of Life and Environmental Sciences, The University of Sydney, Camperdown, NSW, 2006, Australia

    Celso Gaspar Litholdo Jr., Andrew Leigh Eamens & Peter Michael Waterhouse

  2. Citrus Biotechnology Lab, Centro de Citricultura, Instituto Agronômico de Campinas, Cordeirópolis, SP, 13490-000, Brazil

    Celso Gaspar Litholdo Jr.

  3. School of Environmental and Life Sciences, The University of Newcastle, Callaghan, NSW, 2308, Australia

    Andrew Leigh Eamens

  4. Centre for Tropical Crops and Biocommodities, Queensland University of Technology, Brisbane, QLD, 4001, Australia

    Peter Michael Waterhouse

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CGLJ, ALE and PMW conceived and designed the research. CGLJ conducted the experiments. CGLJ and ALE analysed the data. CGLJ, ALE and PMW wrote the manuscript. All authors read and approved the manuscript.

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Correspondence to Celso Gaspar Litholdo Jr..

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This work was funded by the CAPES Foundation, Ministry of Education of Brazil (Process BEX nº 040509-4) for financial support and CGLJ’s scholarship. Special thanks to Dr. Ming-Bo Wang (CSIRO Agriculture, Canberra, Australia) for supplying the F. oxysporum inoculate and bioassay methodology.

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Litholdo, C.G., Eamens, A.L. & Waterhouse, P.M. The phenotypic and molecular assessment of the non-conserved Arabidopsis MICRORNA163/S-ADENOSYL-METHYLTRANSFERASE regulatory module during biotic stress. Mol Genet Genomics 293, 503–523 (2018). https://doi.org/10.1007/s00438-017-1399-9

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