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Diclidophora luscae (Monogenea: Diclidophoridae) in pouting, Trisopterus luscus (Linnaeus, 1758) from the northeast Atlantic; epidemiology, morphology, molecular and phylogenetic analysis

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Abstract

Diclidophora (Monogenea) species are gill parasites with a stenoxenic specificity occurring only in Gadiformes. Epidemiological, morphological, molecular and phylogenetic studies were performed on 594 Diclidophora specimens collected from 213 Trisopterus luscus captured in the northeast Atlantic off the Portuguese coast during 2012, 2013 and 2020. Prevalence, parasite abundance and infection intensity were determined. Positive correlation between fish weight and length and infection intensity was observed. The effects of preservation on the parasite morphological features were studied, highlighting that specimen’s identification should be reinforced by molecular studies. A sequence of D. luscae capelanii from T. capelanus captured in the Mediterranean Sea included in the 28S rDNA molecular analysis was nested within a robust D. luscae clade. Data analysis suggested that this species is in fact D. luscae, which is compatible with T. luscus and T. capelanus. The identity of fish hosts was confirmed by barcoding. For the first time, data on the infection parameters is shown, highlighting the importance of including this parasite in the monitoring plans for a holistic approach with possible effects for the management of pouting resources aiming of attaining sustainable development and biodiversity conservation measures, according to the 14th objective of the 2030 agenda.

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Acknowledgements

The authors are grateful to Marta Gonçalves, to their assistance to obtain the third sample. The authors are grateful to Kenneth MacKenzie for his kind comments and review of the manuscript.

Funding

This research was funded by SANAQUA MAR-02.05.01-FEAMP-0004 and PREVINE PTDC/ASP-PES/29576/2017 projects through national funds. Thanks also are due to FCT/MCTES for the financial support to CESAM (UIDP/50017/2020 + UIDB/50017/2020 + LA/P/0094/2020) and to CIIMAR (UID/QUI/50006/2019, UIDB/50006/2020, UIDP/50006/2020, UIDB/04423/2020 and UIDP/04423/2020), through national funds, and to Project ReNATURE –Valorization of the Natural Endogenous Resources of the Centro Region (Centro2020, Centro-01–0145-FEDER-000007). Raquel Varandas was funded by FCT (SFRH/BD/130172/2017).

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Authors and Affiliations

  1. Pathology Laboratory of Aquatic Animals, Portuguese Institute for the Ocean and Atmosphere, IPMA,IP., Av. Dr. Alfredo Magalhães Ramalho, 6, 1495-165, Lisboa, Portugal

    Paula Ramos & A. Grade

  2. CIIMAR, Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Rua dos Bragas 289, 4050-123, Porto, Portugal

    Paula Ramos

  3. Centre for Functional Ecology-Science for People and the Planet, Department of Life Sciences, University of Coimbra, Coimbra, Portugal

    R. Varandas & I. L. Conceição

  4. Lusófona University, Campo Grande 376, 1749-024, Lisbon, Portugal

    M. M. Oliveira

  5. INESC TEC - INESC Technology and Science and FEUP, University of Porto, Porto, Portugal

    M. M. Oliveira

  6. Faculty of Veterinary Medicine, University of Lisbon - CIISA, Lisbon, Portugal

    G. Alexandre-Pires

  7. Instituto Superior de Agronomia, Universidade de Lisboa, Tapada da Ajuda, 1349-017, Lisbon, Portugal

    F. Rosa

  8. Centro de Estudos do Ambiente e do Mar, CESAM, LA, Faculdade de Ciências, Universidade de Lisboa, Campo Grande, 1749-016, Lisbon, Portugal

    F. Rosa

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  1. Paula Ramos
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  2. R. Varandas
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  4. A. Grade
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  5. M. M. Oliveira
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Correspondence to Paula Ramos.

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Ramos, P., Varandas, R., Conceição, I.L. et al. Diclidophora luscae (Monogenea: Diclidophoridae) in pouting, Trisopterus luscus (Linnaeus, 1758) from the northeast Atlantic; epidemiology, morphology, molecular and phylogenetic analysis. Parasitol Res 121, 2517–2535 (2022). https://doi.org/10.1007/s00436-022-07591-8

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