Abstract
Trichomoniasis—caused by the parasite Trichomonas vaginalis—is associated with a high inflammatory process that may contribute to the risk of suffering from other medical complications. Our study focused on the in vitro interaction of T. vaginalis with human neutrophils because these are the most abundant cells implicated in the characteristic inflammatory process of trichomoniasis. This study showed that T. vaginalis and its surface glycoconjugates (lipophosphoglycan and/or lipoglycan) induced the formation of human neutrophil extracellular traps (NETs). After the trichomonad-neutrophil interaction, parasite integrity was at 32.9%, and the subsequent parasite growth was at 35.2% compared to those of control trophozoites (100%) incubated under the same conditions without neutrophils. In the presence of an antibody against the TLR-4 receptor, DNase I or micrococcal nuclease (MNase), neutrophils reduced the DNA fibres of the NETs and the amount of extracellular DNA, allowing a higher subsequent growth of T. vaginalis, at 52% with the anti-TLR-4 antibody and 62.6% with the enzymes. These results indicated that T. vaginalis induced the formation of extracellular traps by human neutrophils and, because of the interaction with neutrophils and NETs, parasite integrity and growth decreased.
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Acknowledgements
We thank Dr. Patricia Talamás Rohana and Dr. Mireya de la Garza, CINVESTAV, Mexico, for their helpful discussions and suggestions.
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The Consejo Nacional de Ciencia y Tecnologia (CONACYT, project CB-2012-01 182671) and the Universidad de Guanajuato (project 977/2016) provided financial support.
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Ramírez-Ledesma, M.G., Romero-Contreras, Y.J., Rodríguez, M.C. et al. Trichomonas vaginalis triggers neutrophil extracellular traps reducing parasite integrity and growth. Parasitol Res 121, 1355–1367 (2022). https://doi.org/10.1007/s00436-022-07475-x
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DOI: https://doi.org/10.1007/s00436-022-07475-x