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piRNA-3878 targets P450 (CpCYP307B1) to regulate pyrethroid resistance in Culex pipiens pallens

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Abstract

Piwi-interacting RNAs (piRNAs) are a novel class of noncoding single-strand RNAs. They play an important role in the germ cell maintenance, brain development, epigenetic regulation of cancer, and antiviral function. However, little is known about the relationship between the piRNAs and insecticide resistance in mosquitoes. In this study, we reported that piRNA-3878 was related with pyrethroid resistance in Culex pipiens pallens. The expression level of piRNA-3878 was lower in both laboratory and field-collected deltamethrin-resistant (DR) strains. Overexpression of piRNA-3878 increased the susceptibility of the DR strain, while inhibiting the expression of piRNA-3878 in DS strain made the mosquitoes more resistant to deltamethrin. Furthermore, we identified that CpCYP307B1 was the target of piRNA-3878. The mosquito mortality rate was increased after downregulating the expression of CpCYP307B1. These findings revealed that piRNA-3878 could target CpCYP307B1 to regulate pyrethroid resistance.

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Acknowledgements

This work was supported by the National Institutes of Health of the USA (NIH) (Grant No. 2R01AI075746), the National Natural Science Foundation of China (Grant No. 81171610, 81471984, and 81301458), and the Priority Academic Program Development of Jiangsu Higher Education Institutions.

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  1. Wenyun Ye and Xianmiao Liu contributed equally to this work.

Authors and Affiliations

  1. Department of Pathogen Biology, Nanjing Medical University, 101 Longmian Road, Jiangning District, Nanjing, Jiangsu, 211166, China

    Wenyun Ye, Xianmiao Liu, Juxin Guo, Xueli Sun, Yan Sun, Bo Shen, Dan Zhou & Changliang Zhu

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  1. Wenyun Ye
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  2. Xianmiao Liu
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  3. Juxin Guo
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  5. Yan Sun
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  7. Dan Zhou
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Correspondence to Dan Zhou.

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Ye, W., Liu, X., Guo, J. et al. piRNA-3878 targets P450 (CpCYP307B1) to regulate pyrethroid resistance in Culex pipiens pallens . Parasitol Res 116, 2489–2497 (2017). https://doi.org/10.1007/s00436-017-5554-3

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  • DOI: https://doi.org/10.1007/s00436-017-5554-3

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