Abstract
Mosquitoes are the vector of more diseases and cause major health problems like malaria, dengue, chikungunya, and lymphatic filariasis. This article deals with the mosquito larvicidal activity of Chloroxylon swietenia Dc bark extracts against late third instar larvae of Culex quinquefasciatus, Aedes aegypti, and Anopheles stephensi. Methanolic crude extract of Ch. swietenia bark was obtained by soxhlet apparatus and aqueous crude extract by cold percolation method. The range of concentrations of the crude extracts used was 50, 100, 150, 200, and 250 ppm. The mortality and lethal concentration (LC50 and LC90) was calculated after a 24-h exposure period. Both the extracts showed trustworthy larvicidal activity. The larvicidal activity of the methanol extract of Ch. swietenia bark was higher than the aqueous extract, and the LC50 and the LC90 values of the methanol extract were found to be 124.70 and 226.26 μg/ml (Ae. aegypti), 130.57 and 234.67 ppm (Cu. quinquefasciatus), and 137.55 and 246.09 ppm (An. stephensi). The LC50 and the LC90 values of the aqueous extract were found to be 133.10 and 238.93 ppm (Ae. aegypti), 136.45 and 242.47 ppm (Cu. quinquefasciatus), and 139.43 and 248.64 ppm (An. stephensi). No mortality was observed in the control. Methanolic crude extract Ch. swietenia bark shows higher activity against An. stephensi than the other two tested larvae and aqueous extract. The results of the present study propose a possible way for further investigations to find out the active molecule responsible for the larvicidal activity of Ch. swietenia bark extracts.
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Agarwal VK, Sashindran VK (2006) Lymphatic filariasis in India: problems, challenges and new initiatives. Med J Armed Forces India 62:339–362
Arokiyaraj S, Dinesh Kumar V, Elakya V, Kamala T, Sung KP, Ragam M, Saravanan M, Bououdina M, Arasu M, Kovendan K, Vincent S (2015) Biosynthesized silver nanoparticles using floral extract of Chrysanthemum indicum L. potential for malaria vector control. Environ Sci Pollut Res. doi:10.1007/s11356-015-4148-9
Ashraful Alam M, Rowshanul Habib M, Nikkon F, Khalequzzaman M, Rezaul Karim M (2009) Insecticidal activity of root bark of Calotropis gigantea L. against Tribolium castaneum (Herbst). World J Zool 4:90–95
Aziz MA, Bashar K, Chowdhury MMH, Faruque A (2015) Antibacterial, toxicity and larvicidal potentiality of Microcos paniculata barks. PhOL 111:109–120
Essam ASS, Deon C, Mohamed WFY, Hoda AW, Abdel HM (2005) A review of botanical phytochemicals with mosquitocidal potential. Environ Int 31:1149–1166
Finney DJ (1971) Probit analysis, vol 551. Cambridge University Press, London, pp 68–72
Georges K, Jayaprakasam B, Dalavoy SS, Nair MG (2008) Pestmanaging activities of plant extracts and anthraquinones from Cassia nigricans from Burkina Faso. Bioresour Technol 99:2037–2045
Howard AFB, Zhou G, Omlin FX (2007) Malaria mosquito control using edible fish in western Kenya: preliminary findings of a controlled study. BMC Public Health 7:199–204
Jayaprasad B, Sharavanan PS, Karthik K (2014) Chloroxylon swietenia an IUCN red listed plant – medicinal uses. Int J Res Eng Biosci 2:213–217
Jayaraman M, Senthilkumar A, Venkatesalu V (2015) Evaluation of some aromatic plant extracts for mosquito larvicidal potential against Culex quinquefasciatus, Aedes aegypti, and Anopheles stephensi. Parasitol Res 114:1511–1518
Kannathasan K, Senthilkumar A, Chandrasekaran M, Venkatesalu V (2007) Differential larvicidal efficacy of four species of Vitex against Culex quinquefasciatus larvae. Parasitol Res 101:1721–1723
Koul O, Walia S (2009) Comparing impacts of plant extracts and pure allelochemicals and implications for pest control. CAB Rev Perspect Agric Vet Sci Nutr Nat Resour 4:1–30
Kovendan K, Murugan K, Vincent S, Kamalakannan S (2011) Larvicidal efficacy of Jatropha curcas and bacterial insecticide, Bacillus thuringiensis, against lymphatic filarial vector, Culex quinquefasciatus Say. (Diptera: Culicidae). Parasitol Res 109:1251–1257
Krishnappa K, Elumalai K (2012) Larvicidal and ovicidal activities of Chloroxylon swietenia (Rutaceae) essential oils against Spodoptera litura (Lepidoptera: Noctuidae) and their chemical compositions. Int J Curr Res Life Sci 1:003–007
Kumar A, Valecha N, Jain T, Dash AP (2007) Burden of malaria in India: retrospective and prospective view. Am J Trop Med Hyg 77:69–78
Madhu SK, Vijayan VA (2010) Evaluation of the larvicidal efficacy of extracts from three plants and their synergistic action with propoxur against larvae of the filarial vector Culex quinquefasciatus (Say). Toxicol Environ Chem 92:115–126
Maharaj R, Maharaj V, Newmarch M, Crouch NR, Bhagwandin N, Folb PI, Pillay P, Gayaram R (2010) Evaluation of selected South African ethnomedicinal plants as mosquito repellents against the Anopheles arabiensis mosquito in a rodent model. Malar J 9:301
Mavundza EJ, Maharaj R, Chukwujekwu JC, Finnie JF, Van Staden J (2013) Larvicidal activity against Anopheles arabiensis of 10 South African plants that are traditionally used as mosquito repellents. S Afr J Bot 88:86–89
Mohamed Anees A (2008) Larvicidal activity of Ocimum sanctum Linn. (Labiatae) against Aedes aegypti (L.) and Culex quinquefasciatus (Say). Parasitol Res 103:1451–1453
Oliveira PV, Ferreira JC Jr, Moura FS, Lima GS, de Oliveira FM, Oliveira PES, Conserva LM, Giulietti AM, Lemos RPL (2010) Larvicidal activity of 94 extracts from ten plant species of northeastern of Brazil against Aedes aegypti L. (Diptera: Culicidae). Parasitol Res 107:403–407
Rahman SJ, Shama SK, Rajagopal R (1989) Editors: manual on entomological surveillance of vector borne diseases. National Institute of Communicable Diseases. GOI, New Delhi, pp 209
Ravi V (2006) Re-emergence of Chikungunya virus in India. Ind J Med Microbiol 24:83–84
Ravi Kiran S, Sita Devi P (2007) Evaluation of mosquitocidal activity of essential oil and sesquiterpenes from leaves of Chloroxylon swietenia DC. Parasitol Res 101:413–418
Ravi Kiran S, Bhavani K, Sita Devi P, Rajeswara Rao BR, Janardhan Reddy K (2006) Composition and larvicidal activity of leaves and stem essential oils of Chloroxylon swietenia DC against Aedes aegypti and Anopheles stephensi. Bioresour Technol 97:2481–2484
Senthilkumar A, Kannathasan K, Venkatesalu V (2008) Chemical constituents and larvicidal property of the essential oil of Blumea mollis (D. Don) Merr. against Culex quinquefasciatus. Parasitol Res 103:959–962
Sharma P, Mohan L, Srivastava CN (2009) Amaranthus oleracea and Euphorbia hirta: natural potential larvicidal agents against the urban Indian malaria vector, Anopheles stephensi Liston (Diptera: Culicidae). Parasitol Res 106:171–176
Sosan MB, Adewoyin FB, Adewunmi CO (2001) Larvicidal properties of three indigenous plant oils on the mosquito Aedes aegypti. Nigerian J Nat Prod Med 5:30–33
Srivastava VK, Singh SK, Rai M, Singh A (2003) Toxicity of Nerium indicum and Euphorbia royleana lattices against Culex quinquefasciatus mosquito larvae. Nigerian J Nat Prod Med 7:61–64
Veerakumar K, Govindarajan M (2014) Adulticidal properties of synthesized silver nanoparticles using leaf extracts of Feronia elephantum (Rutaceae) against filariasis, malaria, and dengue vector mosquitoes. Parasitol Res. doi:10.1007/s00436-014-4077-4
Venkataswam R, Mohamad MH, Doss A, Ravi TK, Sukumar M (2010) Ethnobotanical study of medicinal plants used by Malasar tribals in Coimbatore District of Tamil Nadu (South India). Asian J Exp Biol Sci 1:387
World Health Organisation (1981) Instruction for determining the susceptibility or resistance of mosquito larvae to insecticides. WHO-VBC 81:1–6
World Health Organization (2008) Dengue and dengue haemorrhagic fever. Media centre fact sheet. WHO, Geneva
World Health Organization (2010) Dengue transmission research in WHO bulletin Zebit CPW (1984): effect of some crude and Azadirachta enriched neem (Azadirachta indica) seed kernel extracts of larvae of Aedes aegypti. Entomol Exp Appl 35:11–16
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One of the author B. Jayaprasad (IF120300) wishes to thank DST INSPIRE Fellowship Govt. of India, New Delhi, for providing financial support to carry out this research. The authors are grateful to the Field Station, Centre for Research in Medical Entomology (ICMR-Government of India), Virudhachalam for providing mosquito egg.
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Balasubramanian, J., Subramanian, S. & Kaliyan, V. Effect of Chloroxylon swietenia Dc bark extracts against Culex quinquefasciatus, Aedes aegypti, and Anopheles stephensi larvae. Parasitol Res 114, 4219–4223 (2015). https://doi.org/10.1007/s00436-015-4658-x
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DOI: https://doi.org/10.1007/s00436-015-4658-x