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Fungi predatory activity on embryonated Toxocara canis eggs inoculated in domestic chickens (Gallus gallus domesticus) and destruction of second stage larvae

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Abstract

The objective of this study was to evaluate the infectivity of Toxocara canis eggs after interacting with isolated nematophagous fungi of the species Duddingtonia flagrans (AC001) and Pochonia chlamydosporia (VC4), and test the predatory activity of the isolated AC001 on T. canis second stage larvae after 7 days of interaction. In assay A, 5000 embryonated T. canis eggs previously in contact with the AC001 and VC4 isolated for 10 days were inoculated into domestic chickens (Gallus gallus domesticus), and then these animals were necropsied to collect material (digested liver, intestine, muscles and lungs) at 3-, 7-, 14-, and 21-day intervals after inoculation. In assay A, the results demonstrated that the prior interaction of the eggs with isolated AC001 and VC4 decreases the amount of larvae found in the collected organs. Difference (p < 0.01) was observed in the medium larvae counts recovered from liver, lung, intestine, and muscle of animals in the treated groups when compared to the animals in the control group. At the end of assay A, a percentage reduction of 87.1 % (AC001) and 84.5 % (VC4) respectively was recorded. In the result of assay B, the isolated AC001 showed differences (p < 0.01) compared to the control group, with a reduction of 53.4 % in the recovery of L2. Through these results, it is justified to mention that prior interaction of embryonated T. canis eggs with the tested fungal isolates were efficient in reducing the development and migration of this parasite, in addition to the first report of proven predatory activity on L2.

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Authors and Affiliations

  1. Department of Parasitology, University of Vila Velha-ES, Vila Velha, ES, CEP: 29102206, Brazil

    Emy Hiura, Aline del Carmen Garcia Lopes, Jeanne Saraiva da Paz, Maylla Garschagen Gava, Mayra Cunha Flecher, Manuela Colares, Filippe Elias de Freitas Soares, Tracy Lacerda & Fabio Ribeiro Braga

  2. Department of Veterinary Medicine, Federal University of Viçosa, Viçosa, MG, CEP: 3657000, Brazil

    Leandro Abreu da Fonseca & Jackson Victor de Araújo

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  1. Emy Hiura
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  2. Aline del Carmen Garcia Lopes
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Correspondence to Fabio Ribeiro Braga.

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Hiura, E., del Carmen Garcia Lopes, A., da Paz, J.S. et al. Fungi predatory activity on embryonated Toxocara canis eggs inoculated in domestic chickens (Gallus gallus domesticus) and destruction of second stage larvae. Parasitol Res 114, 3301–3308 (2015). https://doi.org/10.1007/s00436-015-4553-5

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