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Prokaryotic expression and identification of 3-1E gene of merozoite surface antigen of Eimeria acervulina

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Abstract

Eimeria acervulina was isolated from chicken at Hebei province, China. The gene of merozoite surface antigen 3-1E was amplified and cloned into pET28a(+) vector and then transformed into Escherichia coli BL21 strain. Results showed that 3-1E fusion protein band of about 22 kDa was identified by SDS-PAGE. Western blot analysis indicated that the recombinant protein specifically reacted with E. acervulina polyclonal antibody.

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Acknowledgments

This research was supported by grants from the National Natural Science Foundation of China (grant number: 30571394) and the National Natural Science Foundation of Hebei province (grant number: C2007000523).

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Authors and Affiliations

  1. College of Chinese Traditional Veterinary Science, Hebei Agriculture University, Dingzhou, China

    Yuelan Zhao & Jianyong Wang

  2. College of Animal Science and Technology, Hebei Agriculture University, Baoding, China

    Yuelan Zhao, Junxia Lu, Yongzhan Bao & Jianhua Qin

  3. National Research Center for Wildlife Borne Diseases, Key Laboratory of Animal Ecology and Conservation Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, China

    Chengmin Wang, Yanmin Lu, Ping Xu & Hongxuan He

  4. Department of Life Sciences, Hengshui University, Hengshui, 053000, Hebei, People’s Republic of China

    Yanmin Lu

  5. Department of Zoology, Faculty of Science, Kafr El-Sheikh University, Kafr El-Sheikh, 33516, Egypt

    Said Amer

  6. Animal Husbandry and Veterinary Station of Beijing, Beijing, 100101, China

    Bolin Deng

Authors
  1. Yuelan Zhao
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  2. Chengmin Wang
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  3. Yanmin Lu
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  4. Said Amer
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  7. Junxia Lu
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  9. Bolin Deng
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  10. Hongxuan He
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  11. Jianhua Qin
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Correspondence to Jianhua Qin.

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Yuelan Zhao, Chengmin Wang, and Yanmin Lu contributed equally to this work.

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Zhao, Y., Wang, C., Lu, Y. et al. Prokaryotic expression and identification of 3-1E gene of merozoite surface antigen of Eimeria acervulina . Parasitol Res 109, 1361–1365 (2011). https://doi.org/10.1007/s00436-011-2381-9

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  • DOI: https://doi.org/10.1007/s00436-011-2381-9

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