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The role of microRNA-30c in targeting interleukin 6, as an inflammatory cytokine, in the mesenchymal stem cell: a therapeutic approach in colorectal cancer

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Abstract

Introduction

Colorectal cancer (CRC) is the third most prevalent cancer and the second significant cause of cancer-associated death worldwide. The microRNA-30 is a substantial member of the miRNA family and plays a vital role in expanding several cancers. This microRNA potentially targets interleukin 6 as an inflammatory cytokine in CRC.

Materials and methods

MSCs were isolated and identified from mice bone marrow and then transduced with lentiviruses containing miR-30C. Transfected MSCs were collected to evaluate IL-6 levels, CT-26 cells were also co-cultured with MSCs, and the effect of apoptosis and IL-6 on the supernatant was assessed.

Results

Our result showed the expression of IL-6 mRNA and the level of protein were decreased in the supernatant of miR-30-transduced MSC cells compared to the control group. In addition, the rate of apoptosis was assessed, and the obtained data revealed the induction of apoptosis in CT-26 cells when they are in the vicinity of miR-30c-transduced MSCs.

Discussion and conclusion

We demonstrated that downregulation of miR-30c was significantly correlated with CRC progression and survival. So, the present study elucidated the anticancer effects of miR-30c in CRC and presented a novel target for CRC therapy.

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Authors and Affiliations

  1. Department of Immunology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran

    Mohamad Mahjoor & Samaneh Khorrami

  2. Department of Medical Microbiology, Faculty of Medicine, Shahed University of Medical Sciences, Tehran, Iran

    Hamed Afkhami

  3. Department of Biochemistry, Faculty of Medical, Iran University of Medical Sciences, Tehran, Iran

    Mohammad Najafi

  4. Department of Biochemistry, Islamic Azad University of Najafabad, Esfahan, Iran

    Atieh Nasr

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  1. Mohamad Mahjoor
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  2. Hamed Afkhami
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  3. Mohammad Najafi
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  4. Atieh Nasr
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  5. Samaneh Khorrami
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MM: data collection; HA: data collection; MN: writing manuscript; AN: help to mn for writing manuscript; SK: corresponding author.

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Correspondence to Samaneh Khorrami.

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Mahjoor, M., Afkhami, H., Najafi, M. et al. The role of microRNA-30c in targeting interleukin 6, as an inflammatory cytokine, in the mesenchymal stem cell: a therapeutic approach in colorectal cancer. J Cancer Res Clin Oncol 149, 3149–3160 (2023). https://doi.org/10.1007/s00432-022-04123-w

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  • DOI: https://doi.org/10.1007/s00432-022-04123-w

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