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High seroprevalence of human herpesvirus type 8 infection in males with advanced lung carcinoma

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Abstract

Human herpesvirus type 8 (HHV-8) DNA is consistently found in all types of Kaposi’s sarcoma, which is prevalent in immunocompromised patients. Patients with advanced lung carcinoma often showed immunologic abnormalities, and prevalence of HHV-8 infection is unclear. In this study, blood samples from 109 lung carcinoma patients and 109 age- and sex-matched healthy controls were analyzed for lymphocyte and monocyte counts, and for antibody, DNA, and genotype of HHV-8. Lung carcinoma patients had significantly lower lymphocyte and higher monocyte counts than healthy controls (p < 0.0001, both). HHV-8 seropositivity was more prevalent in lung carcinoma patients (41.3 %), particularly in male patients (50.8 %), than in controls (24.8 %) (p = 0.01 and 0.002, respectively). The seropositivity was also significantly higher in male (50.8 %) than female patients (27.3 %, p = 0.01). Titers of HHV-8 antibody in patients also significantly exceeded those in controls (p = 0.004). Under a higher threshold (antibody titer ≥1:160) which is equivalent to that of enzyme-linked immunosorbent assay, lung carcinoma patients still had higher HHV-8 seropositivity than controls (p = 0.006). Three patients with stage IV lung carcinoma were positive for HHV-8 DNA with K1 gene subtype C3, D1, and E, respectively; they had much lower lymphocyte counts (658 ± 132 µL) than patients positive for HHV-8 antibodies only (1,449 ± 873 µL). The study indicates that lung carcinoma patients, particularly males, have a high seroprevalence of HHV-8. HHV-8 DNA detected in the patients with advanced lung carcinoma may be a result of virus reactivation in the immunocompromised status.

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References

  1. Su CC, Li CF, Liao YL, Lin CN, Lu JJ (2005) Immunohistochemical and molecular assessment of human herpesvirus type 8 in gastrointestinal tumors. J Clin Pathol 58:856–859

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  2. Al-Khader AA, Shaheen FA (2004) Posttransplant complications encountered in renal transplantation in the Middle East. Transplant Proc 36:180–183

    Article  CAS  PubMed  Google Scholar 

  3. Rady PL, Hodak E, Yen A, Memar O, Trattner A, Feinmesser M, David M, Hudnall SD, Tyring SK (1998) Detection of human herpesvirus-8 DNA in Kaposi’s sarcomas from iatrogenically immunosuppressed patients. J Am Acad Dermatol 38:429–437

    Article  CAS  PubMed  Google Scholar 

  4. Fiuza C, Salcedo M, Clemente G, Tellado JM (2000) In vivo neutrophil dysfunction in cirrhotic patients with advanced liver disease. J Infect Dis 182:526–533

    Article  CAS  PubMed  Google Scholar 

  5. Lombardo L, Capaldi A, Poccardi G, Vineis P (1995) Peripheral blood CD3 and CD4 T-lymphocyte reduction correlates with severity of liver cirrhosis. Int J Clin Lab Res 25:153–156

    Article  CAS  PubMed  Google Scholar 

  6. Rimola A, Soto R, Bory F, Arroyo V, Piera C, Rodes J (1984) Reticuloendothelial system phagocytic activity in cirrhosis and its relation to bacterial infections and prognosis. Hepatology 4:53–58

    Article  CAS  PubMed  Google Scholar 

  7. Chou AL, Huang WW, Tsao SM, Li CT, Su CC (2008) Human herpesvirus type 8 in patients with cirrhosis: correlation with sex, alcoholism, hepatitis B virus, disease severity, and thrombocytopenia. Am J Clin Pathol 130:231–237

    Article  PubMed  Google Scholar 

  8. Chou AL, Huang WW, Lin MN, Su CC (2010) Human herpesvirus type 8 in patients with cirrhosis independent of thrombocytopenia. J Clin Pathol 63:254–258

    Article  CAS  PubMed  Google Scholar 

  9. Cadranel J, Naccache J, Wislez M, Mayaud C (1999) Pulmonary malignancies in the immunocompromised patient. Respiration 66:289–309

    Article  CAS  PubMed  Google Scholar 

  10. Pluygers E, Sadowska A, Chyczewski L, Niklinski J, Niklinska W, Chyczewska E (2001) The impact of immune responses on lung cancer and the development of new treatment modalities. Lung Cancer 34(Suppl 2):S71–S77

    Article  PubMed  Google Scholar 

  11. Reckzeh B, Merte H, Pfluger KH, Pfab R, Wolf M, Havemann K (1996) Severe lymphocytopenia and interstitial pneumonia in patients treated with paclitaxel and simultaneous radiotherapy for non-small-cell lung cancer. J Clin Oncol 14:1071–1076

    CAS  PubMed  Google Scholar 

  12. Fokkema E, Groen HJ, Bauer J, Uges DR, Weil C, Smith IE (1999) Phase II study of oral platinum drug JM216 as first-line treatment in patients with small-cell lung cancer. J Clin Oncol 17:3822–3827

    CAS  PubMed  Google Scholar 

  13. Koukourakis MI, Giatromanolaki A, Schiza S, Kakolyris S, Georgoulias V (1999) Concurrent twice-a-week docetaxel and radiotherapy: a dose escalation trial with immunological toxicity evaluation. Int J Radiat Oncol 43:107–114

    Article  CAS  Google Scholar 

  14. Robert F, Childs HA, Spencer SA, Redden DT, Hawkins MM (1999) Phase I/IIa study of concurrent paclitaxel and cisplatin with radiation therapy in locally advanced non-small cell lung cancer: analysis of early and late pulmonary morbidity. Semin Radiat Oncol 9(2 Suppl 1):136–147

    CAS  PubMed  Google Scholar 

  15. Greene FL, Page DL, Fleming ID, Fritz AG, Balch CM, Haller DG, Morrow M (2002) American joint committee on cancer (AJCC): cancer staging manual, 6th edn. Springer, New York

    Book  Google Scholar 

  16. O’Keefe SJ, El-Zayadi AR, Carraher TE, Davis M (1980) Malnutrition and immuno-incompetence in patients with liver disease. Lancet 2:615–617

    Article  PubMed  Google Scholar 

  17. Muller A, Franzen C, Klussmann P, Wagner M, Diehl V, Fatkenheuer G, Salzberger B, Ablashi DV, Krueger GR (2000) Human herpesvirus type 8 in HIV-infected patients with interstitial pneumonitis. J Infect 40:242–247

    Article  CAS  PubMed  Google Scholar 

  18. Cook PM, Whitby D, Calabro ML, Luppi M, Kakoola DN, Hjalgrim H, Ariyoshi K, Ensoli B, Davison AJ, Schulz TF (1999) Variability and evolution of Kaposi’s sarcoma-associated herpesvirus in Europe and Africa, the International Collaborative Group. AIDS 13:1165–1176

    Article  CAS  PubMed  Google Scholar 

  19. Meng YX, Sata T, Stamey FR, Voevodin A, Katano H, Koizumi H, Deleon M, De Cristofano MA, Galimberti R, Pellett PE (2001) Molecular characterization of strains of Human herpesvirus 8 from Japan, Argentina and Kuwait. J Gen Virol 82(Pt 3):499–506

    CAS  PubMed  Google Scholar 

  20. Su CC, Tsai JP, Lin MN, Hsieh TC, Tseng RC, Chu TY (2013) High seroprevalence of human herpesvirus type 8 in patients with end-stage renal disease in Taiwan. J Clin Virol 58:89–93

    Article  CAS  PubMed  Google Scholar 

  21. Masotti A, Morandini G, Ortolani R, Fumagalli L (1998) Phase-II randomized study of pre-operative IL-2 administration in operable NSCLC. Lung Cancer 20:191–202

    Article  CAS  PubMed  Google Scholar 

  22. Reinheimer C, Allwinn R, Sturmer M (2011) Do fewer cases of Kaposi’s sarcoma in HIV-infected patients reflect a decrease in HHV8 seroprevalence? Med Microbiol Immun 200:161–164

    Article  Google Scholar 

  23. Wang YF, Lee SB, Cheng LC, Tai MH, Su IJ (2002) Detection of serum antibodies to three different recombinant antigens of human herpesvirus 8 by immunoblotting: seroprevalence studies in Taiwan. Clin Chim Acta 320:37–42

    Article  CAS  PubMed  Google Scholar 

  24. Brouchet L, Valmary S, Dahan M, Didier A, Galateau-Salle F, Brousset P, Degano B (2005) Detection of oncogenic virus genomes and gene products in lung carcinoma. Br J Cancer 92:743–746

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  25. Woodberry T, Suscovich TJ, Henry LM, Martin JN, Dollard S, O’Connor PG, Davis JK, Osmond D, Lee TH, Kedes DH, Khatri A, Lee J, Walker BD, Scadden DT, Brander C (2005) Impact of Kaposi sarcoma-associated herpesvirus (KSHV) burden and HIV coinfection on the detection of T cell responses to KSHV ORF73 and ORF65 proteins. J Infect Dis 192:622–629

    Article  CAS  PubMed  Google Scholar 

  26. Azadmanesh K, Norouzfar ZS, Sohrabi A, Safaie-Naraghi Z, Moradi A, Yaghmaei P, Naraghi MM, Arashkia A, Eslamifar A (2012) Characterization of human herpes virus 8 genotypes in Kaposi’s sarcoma patients in Tehran, Iran. Int J Mol Epidemiol Genet 3:144–152

    PubMed Central  CAS  PubMed  Google Scholar 

  27. Cassar O, Charavay F, Bassot S, Plancoulaine S, Grangeon JP, Laumond-Barny S, Martin PM, Chanteau S, Gessain A (2012) Divergent KSHV/HHV-8 subtype D strains in New Caledonia and Solomon Islands, Melanesia. J Clin Virol 53:214–218

    Article  PubMed  Google Scholar 

  28. Vuckovic-Dekic L, Susnjar S, Stanojevic-Bakic N, Radosavljevic D (1999) Radio- and chemotherapy variably affect the general immunocompetence of lung cancer patients. Panminerva Med 41:129–133

    CAS  PubMed  Google Scholar 

  29. Okabe T, Yasukawa K (1990) A human monocyte growth factor produced by lung cancer cells. Cancer Res 50:3863–3865

    CAS  PubMed  Google Scholar 

  30. Kedes DH, Ganem D, Ameli N, Bacchetti P, Greenblatt R (1997) The prevalence of serum antibody to human herpesvirus 8 (Kaposi sarcoma-associated herpesvirus) among HIV-seropositive and high-risk HIV-seronegative women. JAMA 277:478–481

    Article  CAS  PubMed  Google Scholar 

  31. Su CC, Lu JJ, Perng CL, Yu FT, Chiu CH (2006) Evolution of human herpesvirus type 8-associated gastric Kaposi’s sarcoma following corticosteroid treatment for asthma. J Formos Med Assoc 105:155–159

    Article  PubMed  Google Scholar 

  32. Parravicini C, Olsen SJ, Capra M, Poli F, Sirchia G, Gao SJ, Berti E, Nocera A, Rossi E, Bestetti G, Pizzuto M, Galli M, Moroni M, Moore PS, Corbellino M (1997) Risk of Kaposi’s sarcoma-associated herpes virus transmission from donor allografts among Italian posttransplant Kaposi’s sarcoma patients. Blood 90:2826–2829

    CAS  PubMed  Google Scholar 

  33. Frances C, Marcelin AG, Legendre CH, Chevret S, Dussaix E, Lejeune J, Euvrard S, Bigorie A, Schulz TF, Agbalika F, Lebbe C (2009) Skin and Organ Transplantation Group of the French Society of Dermatology. The impact of preexisting or acquired Kaposi sarcoma herpesvirus infection in kidney transplant recipients on morbidity and survival. Am J Transplant 9:2580–2586

    Article  CAS  PubMed  Google Scholar 

  34. de Sanjose S, Mbisa G, Perez-Alvarez S, Benavente Y, Sukvirach S, Hieu NT, Shin HR, Anh PT, Thomas J, Lazcano E, Matos E, Herrero R, Munoz N, Molano M, Franceschi S, Whitby D (2009) Geographic variation in the prevalence of Kaposi sarcoma-associated herpesvirus and risk factors for transmission. J Infect Dis 199:1449–1456

    Article  PubMed  Google Scholar 

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Acknowledgments

This study was supported by grants DTCRD94(02)-08 and DTCRD96(2)-02 from Buddhist Dalin Tzu Chi Hospital, Chiayi, Taiwan.

Conflict of interest

None of the authors have any conflicts of interest to report.

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Authors and Affiliations

  1. Institute of Medical Sciences, Tzu Chi University, Hualien, Taiwan

    Cheng-Chuan Su & Tang-Yuan Chu

  2. Departments of Clinical Pathology and Anatomic Pathology, Buddhist Dalin Tzu Chi Hospital, Chiayi, Taiwan

    Cheng-Chuan Su

  3. Departments of Laboratory Medicine and Pathology, School of Medicine, Tzu Chi University, Hualien, Taiwan

    Cheng-Chuan Su

  4. Department of Chest Medicine, Buddhist Dalin Tzu Chi Hospital, Chiayi, Taiwan

    Chun-Liang Lai

  5. School of Medicine, Tzu Chi University, Hualien, Taiwan

    Chun-Liang Lai

  6. Institute of Microbiology and Immunology, Chung Shan Medical University, Taichung, Taiwan

    Shih-Ming Tsao

  7. Sections of Infectious Diseases and Chest Medicine, Department of Internal Medicine, Chung Shan Medical University Hospital, Taichung, Taiwan

    Shih-Ming Tsao

  8. Department of Family Medicine, Buddhist Dalin Tzu Chi Hospital, Chiayi, Taiwan

    Ming-Nan Lin

  9. Department of Obstetrics and Gynecology, Buddhist Tzu Chi Medical Center, Hualien, Taiwan

    Tang-Yuan Chu

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  1. Cheng-Chuan Su
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  2. Chun-Liang Lai
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Correspondence to Cheng-Chuan Su or Tang-Yuan Chu.

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Su, CC., Lai, CL., Tsao, SM. et al. High seroprevalence of human herpesvirus type 8 infection in males with advanced lung carcinoma. Med Microbiol Immunol 204, 177–183 (2015). https://doi.org/10.1007/s00430-014-0352-3

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  • DOI: https://doi.org/10.1007/s00430-014-0352-3

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