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Differential distribution of Y-box-binding protein 1 and cold shock domain protein A in developing and adult human brain

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An Erratum to this article was published on 18 June 2014

Abstract

The two cold shock domain containing proteins, Y-box-binding protein-1 and cold shock domain protein A were immunolocalized in developing and adult human brain. With the exception of a small population of hypothalamic astrocytes, brain Y-box-binding protein-1 was predominantly found in multiple neurons in the mature human CNS, which might be related to its involvement in neurotransmission and other neuron-associated functions. Cold shock domain protein A was typically observed in astrocytes, oligodendrocytes, choroid plexus epithelia and nerve fibers. However, in circumscribed brain regions as hypothalamus, habenula, and cerebellum, this protein was also expressed in neurons. In the prenatal brain, both proteins were found to be abundantly expressed in radial glial cells, neuroblasts and neurons, which might be an anatomical correlate of the proposed roles of both proteins in cell proliferation and differentiation. In addition, Y-box-binding protein-1 was identified in cultured, lipopolysaccharide-stimulated microglial cells, which underscores its putative role as a mediator in immune and inflammatory processes.

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Abbreviations

Aka:

Also known as

CD5:

Cluster of differentiation (glycoprotein) 5

Cgl:

Corpus geniculatum laterale

CNS:

Central nervous system

CSD:

Cold shock domain

DbPA:

DNA binding protein A

DbPB:

DNA binding protein B

DMEM:

Dulbecco’s modified Eagle medium

DNA:

Deoxyribonucleic acid

DIV:

Days in vitro (Days in culture)

FCS:

Fetal calf serum

GFAP:

Glial fibrillary acidic protein

GnRH:

Gonadotropin releasing hormone

LPS:

Lipopolysaccharide

mRNPs:

Messenger ribonucleoprotein particles

MSY4:

Murine sequence-specific RNA binding protein

NSEP-1:

Nuclease sensitive element binding protein 1

PVN:

Paraventricular nucleus

RNA:

Ribonucleic acid

rpm:

Rotations per minute

SON:

Supraoptic nucleus

YB-1:

Y-box-binding protein 1

ZONAB:

Zonula occludens 1 (ZO-1)-associated nucleic acid binding protein

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Acknowledgement

The authors are grateful for the skilled technical assistance of K. Paelchen, B. Jerzykiewicz and I. Bück.

Conflict of interest

The authors declare that they have no conflict of interest. P. Mertens is one of the founding members of Antibodies Online.

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Authors and Affiliations

  1. Department of Psychiatry, Medical School, Otto-von-Guericke University, Leipziger Str. 44, 39120, Magdeburg, Germany

    Hans-Gert Bernstein, Henrik Dobrowolny, Johann Steiner & Bernhard Bogerts

  2. Department of Nephrology and Hypertension, Diabetes and Endocrinology, Otto-von-Guericke University, Magdeburg, Germany

    Jonathan A. Lindquist, Sabine Brandt & Peter R. Mertens

  3. Faculty of Medicine, Institute of Biochemistry and Cell Biology, Otto-von-Guericke University, Magdeburg, Germany

    Gerburg Keilhoff

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  1. Hans-Gert Bernstein
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  2. Jonathan A. Lindquist
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  3. Gerburg Keilhoff
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  4. Henrik Dobrowolny
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  5. Sabine Brandt
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  6. Johann Steiner
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  7. Bernhard Bogerts
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Correspondence to Hans-Gert Bernstein.

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Bernstein, HG., Lindquist, J.A., Keilhoff, G. et al. Differential distribution of Y-box-binding protein 1 and cold shock domain protein A in developing and adult human brain. Brain Struct Funct 220, 2235–2245 (2015). https://doi.org/10.1007/s00429-014-0786-9

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  • DOI: https://doi.org/10.1007/s00429-014-0786-9

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